Addition of Calcium to Euro-Collins Solution Is Essential for 24-Hour Preservation of the Vasculature Richard Ingemansson, MD, PhD, Ramunas Bolys, MD,

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Presentation transcript:

Addition of Calcium to Euro-Collins Solution Is Essential for 24-Hour Preservation of the Vasculature Richard Ingemansson, MD, PhD, Ramunas Bolys, MD, Algimantas Budrikis, MD, Anna Lindgren, BSc, Trygve Sjöberg, PhD, Stig Steen, MD, PhD The Annals of Thoracic Surgery Volume 63, Issue 2, Pages 408-413 (February 1997) DOI: 10.1016/S0003-4975(96)00897-1

Fig. 1 Contractile response to the thromboxane analogue U-46619 (top) and the maximum endothelium-dependent relaxation elicited by acetylcholine in rat aorta precontracted by U-46619 (bottom). Bars show the results from fresh controls and from vessels preserved for 24 hours in genuine Euro-Collins solution at 4°C and in Euro-Collins solution with added calcium. Each bar represents the mean ± standard error of the mean; n = 10 rats in all groups. (A = endothelium-dependent relaxation could not be investigated because the vessels lost almost all ability to contract; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.) The Annals of Thoracic Surgery 1997 63, 408-413DOI: (10.1016/S0003-4975(96)00897-1)

Fig. 2 Transmission electron microscopy of endothelial cells investigated immediately after harvesting (A) and after 24 hours’ storage in genuine Euro-Collins solution (B) and in Euro-Collins solution with 1.5 mmol/L added calcium (C). The homogeneous bandlike structures next to the endothelial cells represent the internal elastic lamina, and arrows indicate the endothelial cells. (All ×8,320 before 35% reduction.) The Annals of Thoracic Surgery 1997 63, 408-413DOI: (10.1016/S0003-4975(96)00897-1)

Fig. 3 Transmission electron microscopy of the nuclei of smooth muscle cells investigated immediately after harvesting (A) and after 24 hours’ storage in genuine Euro-Collins solution (B) and in Euro-Collins solution with 1.5 mmol/L added calcium (C). The arrows indicate the smooth muscle nuclei. (All ×10,560 before 35% reduction.) The Annals of Thoracic Surgery 1997 63, 408-413DOI: (10.1016/S0003-4975(96)00897-1)

Fig. 4 Transmission electron microscopy of mitochondria investigated immediately after harvesting (A) and after 24 hours’ storage in genuine Euro-Collins solution (B) and in Euro-Collins solution with 1.5 mmol/L added calcium (C). The arrows indicate a typical mitochondrion. (All ×45,600 before 35% reduction.) The Annals of Thoracic Surgery 1997 63, 408-413DOI: (10.1016/S0003-4975(96)00897-1)