Sensitivity of the ViroSeq HIV-1 Genotyping System for Detection of the K103N Resistance Mutation in HIV-1 Subtypes A, C, and D Jessica D. Church, Dana.

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Sensitivity of the ViroSeq HIV-1 Genotyping System for Detection of the K103N Resistance Mutation in HIV-1 Subtypes A, C, and D Jessica D. Church, Dana Jones, Tamara Flys, Donald Hoover, Natalia Marlowe, Shu Chen, Chanjuan Shi, James R. Eshleman, Laura A. Guay, J. Brooks Jackson, Newton Kumwenda, Taha E. Taha, Susan H. Eshleman The Journal of Molecular Diagnostics Volume 8, Issue 4, Pages 430-432 (September 2006) DOI: 10.2353/jmoldx.2006.050148 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Detection of the K103N mutation by the ViroSeq system in samples with different levels of K103N-containing HIV-1 variants. Samples with subtype A, C, and D were analyzed with the LigAmp assay to determine the percentage of HIV-1 variants in the viral population that had the K103N mutation. Samples with >1% K103N detected in the LigAmp assay were divided into categories for each subtype with >20% K103N, >10% but ≤20% K103N, >5% but ≤10% K103N, and >1% but ≤5% K103N. The graph indicates the percentage of samples in each category that had K103N detected using the ViroSeq system. The number of samples tested in each sample subset is indicated below each bar. The Journal of Molecular Diagnostics 2006 8, 430-432DOI: (10.2353/jmoldx.2006.050148) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions