Stage-specific differences in secretory profile of mesenchymal stromal cells (MSCs) subjected to early- vs late-stage OA synovial fluid  A. Gómez-Aristizábal, A. Sharma, M.A. Bakooshli, M. Kapoor, P.M. Gilbert, S. Viswanathan, R. Gandhi  Osteoarthritis and Cartilage  Volume 25, Issue 5, Pages 737-741 (May 2017) DOI: 10.1016/j.joca.2016.11.010 Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Experimental plan diagram: To identify the secretome of MSCs in OA SFs and possible differential effects of SFs from different OA stages, a single-donor MSC was cultured in OA SFs and later the conditioned SFs were tested using a 64-plex immunoassays, from which four cytokines were selected for further analysis with MSCs from three donors using a 4-plex immunoassay. Osteoarthritis and Cartilage 2017 25, 737-741DOI: (10.1016/j.joca.2016.11.010) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Selected cytokines secreted by MSCs in early- and late-stage OA SFs. Cytokines are shown as fold change from the baseline MSC secretion (dotted line) in DMEM with 0.5% FBS: with IL6 at 1513 [1291, 1843] pg/mL; CCL2 at 1946 [1706, 2084] pg/mL; CXCL8 at 71 [46, 387] pg/mL; and CXCL12 at 199 [197, 279] pg/mL. Individual values are shown with median and ranges indicate interquartile range. P values shown indicate comparison between the variation of cytokine levels in early- (n = 12) vs late-stage OA (n = 12) SF after exposure to MSCs. Osteoarthritis and Cartilage 2017 25, 737-741DOI: (10.1016/j.joca.2016.11.010) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions