Knockdown of Rpn11 inhibits TBSV accumulation in plants.

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Knockdown of Rpn11 inhibits TBSV accumulation in plants. Knockdown of Rpn11 inhibits TBSV accumulation in plants. (A) Silencing of RPN11 expression was induced based on the TRV vector carrying RPN11 sequence or the N-terminal half of GFP (negative control). The upper silenced leaves of N. benthamiana were sap inoculated with infectious TBSV 7 days after agroinfiltration, followed by analysis of the accumulation of TBSV RNAs by Northern blotting (top) 3 days after inoculation. The rRNA was used as a loading control and is shown in an agarose gel stained with ethidium bromide (bottom). (B) The upper silenced leaves (7 days after the original agroinfiltration) were agroinfiltrated to express CNV from the 35S promoter. Total RNA was extracted from leaves 3 days after agroinfiltration with the CNV plasmid, followed by Northern blotting. See further details in the description for panel A. K. Reddisiva Prasanth et al. J. Virol. 2015;89:2750-2763