Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9

Slides:



Advertisements
Similar presentations
Interaction between Rice stripe virus Disease-Specific Protein and Host PsbP Enhances Virus Symptoms Lingfang Kong, Jianxiang Wu, Lina Lu, Yi Xu, Xueping.
Advertisements

Genome Engineering with CRISPR-Cas9 in the Mosquito Aedes aegypti
Glow in the Dark: Fluorescent Proteins as Cell and Tissue-Specific Markers in Plants Wenzislava Ckurshumova, Adriana E. Caragea, Rochelle S. Goldstein,
Rice OsGL1-1 Is Involved in Leaf Cuticular Wax and Cuticle Membrane Bao-Xiang Qin, Ding Tang, Jian Huang, Ming Li, Xin-Ru Wu, Li-Li Lu, Ke-Jian Wang, Heng-Xiu.
A High-Density SNP Genotyping Array for Rice Biology and Molecular Breeding Haodong Chen, Weibo Xie, Hang He, Huihui Yu, Wei Chen, Jing Li, Renbo Yu, Yue.
A Novel Protein RLS1 with NB–ARM Domains Is Involved in Chloroplast Degradation during Leaf Senescence in Rice Jiao Bin-Bin, Wang Jian-Jun, Zhu Xu-Dong,
Expression, Imprinting, and Evolution of Rice Homologs of the Polycomb Group Genes Luo Ming, Platten Damien, Chaudhury Abed, Peacock W.J., Dennis Elizabeth.
Structure and Activity of Strigolactones: New Plant Hormones with a Rich Future Binne Zwanenburg, Tomáš Pospíšil Molecular Plant Volume 6, Issue 1, Pages.
ABH2 Couples Regulation of Ribosomal DNA Transcription with DNA Alkylation Repair Pishun Li, Shuman Gao, Lina Wang, Fang Yu, Jialun Li, Chuangui Wang,
2015 Alzheimer's disease facts and figures Alzheimer's & Dementia: The Journal of the Alzheimer's Association Volume 11, Issue 3, Pages (March.
Association of Matrix Metalloproteinase 9 C-1562T Polymorphism with Genetic Susceptibility to Myocardial Infarction: A Meta-Analysis Zhang Juan, MD, Zhang.
FERONIA Is a Key Modulator of Brassinosteroid and Ethylene Responsiveness in Arabidopsis Hypocotyls Deslauriers Stephen D., Larsen Paul B. Molecular Plant.
Rab-A1c GTPase Defines a Population of the Trans-Golgi Network that Is Sensitive to Endosidin1 during Cytokinesis in Arabidopsis Xingyun Qi, Huanquan Zheng.
Comprehensive Sequence and Whole-Life-Cycle Expression Profile Analysis of the Phosphate Transporter Gene Family in Rice Liu Fang, Chang Xiao-Jian, Ye.
Yuming Lu, Jian-Kang Zhu  Molecular Plant 
Rice Functional Genomics Research: Past Decade and Future
Volume 9, Issue 2, Pages (February 2016)
Volume 8, Issue 12, Pages (December 2015)
IPA1: A New “Green Revolution” Gene?
Volume 9, Issue 11, Pages (November 2016)
Volume 9, Issue 12, Pages (December 2016)
Yang Lei, Li Lu, Hai-Yang Liu, Sen Li, Feng Xing, Ling-Ling Chen 
Harnessing the Potential of the Tea Tree Genome
Volume 10, Issue 9, Pages (September 2017)
Volume 10, Issue 3, Pages (March 2017)
Volume 8, Issue 11, Pages (November 2015)
Mutant Resources for the Functional Analysis of the Rice Genome
AGEseq: Analysis of Genome Editing by Sequencing
Chapter 24 Genomics and DNA Sequencing
Volume 8, Issue 2, Pages (February 2015)
Volume 26, Issue 2, Pages (February 2018)
Volume 11, Issue 4, Pages (April 2018)
Volume 6, Issue 3, Pages (May 2013)
Volume 11, Issue 1, Pages (January 2018)
Volume 10, Issue 7, Pages (July 2017)
H3K36 Methylation Is Involved in Promoting Rice Flowering
Revolutionize Genetic Studies and Crop Improvement with High-Throughput and Genome-Scale CRISPR/Cas9 Gene Editing Technology  Ning Yang, Rongchen Wang,
Volume 130, Issue 6, (September 2007)
Quantitative Disease Resistance: Dissection and Adoption in Maize
Volume 8, Issue 9, Pages (September 2015)
Volume 11, Issue 4, Pages (April 2018)
Volume 9, Issue 9, Pages (September 2016)
Double the Genome, Double the Fun: Genome Duplications in Angiosperms
Volume 11, Issue 3, Pages (March 2018)
PlantcircBase: A Database for Plant Circular RNAs
Volume 9, Issue 1, Pages (January 2016)
Volume 9, Issue 9, Pages (September 2016)
A Sequencing-Based Linkage Map of Cucumber
Transforming CO2 by Stabilizing the Labile Product
Expanding the Range of CRISPR/Cas9 Genome Editing in Rice
Volume 10, Issue 1, Pages (January 2017)
AtPRMT5 Regulates Shoot Regeneration through Mediating Histone H4R3 Dimethylation on KRPs and Pre-mRNA Splicing of RKP in Arabidopsis  Hui Liu, Xu Ma,
Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System
Volume 10, Issue 5, Pages (May 2017)
Targeted Genome Editing in Genes and cis-Regulatory Regions Improves Qualitative and Quantitative Traits in Crops  Xitao Li, Yongyao Xie, Qinlong Zhu,
A Welcome Proposal to Amend the GMO Legislation of the EU
Yi Song, Chuangwei Yang, Shan Gao, Wei Zhang, Lin Li, Benke Kuai 
Volume 5, Issue 4, Pages (July 2012)
Turnip Yellow Mosaic Virus P69 Interacts with and Suppresses GLK Transcription Factors to Cause Pale-Green Symptoms in Arabidopsis  Fangrui Ni, Liang.
Shaopei Gao, Chengcai Chu  Molecular Plant 
Volume 6, Issue 5, Pages (September 2013)
Volume 10, Issue 7, Pages (July 2017)
Volume 6, Issue 4, Pages (July 2013)
Volume 10, Issue 9, Pages (September 2017)
Volume 134, Issue 6, (September 2008)
The bHLH Transcription Factors MYC2, MYC3, and MYC4 Are Required for Jasmonate- Mediated Inhibition of Flowering in Arabidopsis  Houping Wang, Yang Li,
Comparative Population Genomics Reveals Strong Divergence and Infrequent Introgression between Asian and African Rice  Xuehui Huang, Qiang Zhao, Bin Han 
Volume 2, Issue 1, Pages (January 2009)
Epidemiologic data of renal diseases from a single unit in China: Analysis based on 13,519 renal biopsies  Lei-Shi Li, Zhi-Hong Liu  Kidney International 
H2A.Z at the Core of Transcriptional Regulation in Plants
Presentation transcript:

Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9 Xiangbing Meng, Hong Yu, Yi Zhang, Fengfeng Zhuang, Xiaoguang Song, Songsong Gao, Caixia Gao, Jiayang Li  Molecular Plant  Volume 10, Issue 9, Pages 1238-1241 (September 2017) DOI: 10.1016/j.molp.2017.06.006 Copyright © 2017 The Author Terms and Conditions

Figure 1 Construction of a Genome-Wide Mutant Library in Rice Using CRISPR/Cas9. (A) Schematic illustration of the CRISPR/Cas9 rice library construction. (B) Results of large-scale sequencing of the sgRNA library. (C) Morphological phenotypes of the CRISPR/Cas9 rice mutants. The T1 mutant plants showing abnormal phenotypes growing in normal field conditions in Beijing. Bar, 24 cm. Molecular Plant 2017 10, 1238-1241DOI: (10.1016/j.molp.2017.06.006) Copyright © 2017 The Author Terms and Conditions