Methylation of PTEN as a Prognostic Factor in Malignant Melanoma of the Skin  Christoph Lahtz, René Stranzenbach, Eckhard Fiedler, Peter Helmbold, Reinhard H. Dammann  Journal of Investigative Dermatology  Volume 130, Issue 2, Pages 620-622 (February 2010) DOI: 10.1038/jid.2009.226 Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Influence of PTEN hypermethylation on cumulative survival and methylation analysis of PTEN promoter. (a) Overall survival stratified by PTEN methylation (n=200, P=0.035, confidence interval: Supplementary Table S4). (b) DNA isolated from different regions (a, b, and c) from primary melanomas (cases 161 and 135), in vitro methylated DNA (meth.), and unmethylated controls (unmeth.) was treated with bisulfite and amplified by methylation-specific PCR. Methylation (m)- and unmethylation (u)-specific PCR products of PTEN (117 and 135bp, respectively) were resolved on a 2% TBE gel, together with a 100-bp ladder (marker). Journal of Investigative Dermatology 2010 130, 620-622DOI: (10.1038/jid.2009.226) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions