Volume 11, Issue 4, Pages 623-626 (April 2018) Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9-Guided Hyperactive hAID Mutant  Bin Ren, Fang Yan, Yongjie Kuang, Na Li, Dawei Zhang, Xueping Zhou, Honghui Lin, Huanbin Zhou  Molecular Plant  Volume 11, Issue 4, Pages 623-626 (April 2018) DOI: 10.1016/j.molp.2018.01.005 Copyright © 2018 The Authors Terms and Conditions

Figure 1 Targeted Base Editing in Rice Using the rBE5 and rBE9 Systems. (A) Schematic of rBE5-mediated C to U conversion on the non-complementary strand of DNA. (B) Constructs of the rBE5 system used to induce nucleotide changes in rice protoplasts or transgenic plants. (C) The target site of pi-d2 gene in rice. (D) Representative Sanger sequencing chromatogram of the rBE5-edited pi-d2 alleles with the target G changed in T0 transgenic rice line #3. (E) Efficiency of rBE5- versus rBE3-mediated gene correction of pi-d2 in T0 transgenic rice lines. (F) The target site of OsFLS2 gene in rice. (G) Representative Sanger sequencing chromatogram of the rBE5-edited OsFLS2 alleles with anticipated C > A conversion in T0 transgenic rice line #21. (H) Efficiency of the rBE5 system in base editing OsFLS2 in T0 transgenic rice lines. (I) Sequencing results of the rBE5-induced OsFLS2 mutations in T0 transgenic rice lines. (J) Construct of the rBE9 system used to induce nucleotide changes in rice transgenic plants. (K) Efficiencies and ratios of allelic mutations caused by the rBE3 and rBE9 systems. (L) Base editing efficiencies of the rBE3 and rBE9 systems at the target C in different sequence context. For (C, F, and I), the PAM sequences, the putative target bases in the activity window, and the detected nucleotide/corresponding amino acids are highlighted in green, red, and blue, respectively. For (D and G), nucleotide mutations are underlined in the sequencing chromatograms. Molecular Plant 2018 11, 623-626DOI: (10.1016/j.molp.2018.01.005) Copyright © 2018 The Authors Terms and Conditions