Identification of MGB1 as a Marker in the Differential Diagnosis of Lung Tumors in Patients with a History of Breast Cancer by Analysis of Publicly Available.

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Identification of MGB1 as a Marker in the Differential Diagnosis of Lung Tumors in Patients with a History of Breast Cancer by Analysis of Publicly Available SAGE Data Takaomi Koga, Yoshitsugu Horio, Tetsuya Mitsudomi, Takashi Takahashi, Yasushi Yatabe The Journal of Molecular Diagnostics Volume 6, Issue 2, Pages 90-95 (May 2004) DOI: 10.1016/S1525-1578(10)60495-3 Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Expression analysis of potential breast-specific molecules (A) reveals that bulk tissue of normal lung (lane 1) expressed SBEM and PDEF, but not MGB1. Detailed examination using laser capture microdissection (lanes 2 to 4) demonstrated that bronchial surface epithelium and bronchial gland cells, but not the peripheral lung, were the source of the expression. In tumors (B), MGB1 expression was specific to breast cancers (B, lanes 9 to 15), with the notable exception of small-cell lung carcinomas (B, lane 8). DW indicates distilled water. The Journal of Molecular Diagnostics 2004 6, 90-95DOI: (10.1016/S1525-1578(10)60495-3) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Practical application of MGB1 to an 80-year-old woman, who had a history of breast cancer 13 years previously. A lung tumor (A) and a lymph node of her left neck (B) were biopsied. In the lung tumor specimen, a few degenerated atypical cells are seen in the background fibrosis. Although the atypical cells are suggestive of an adenocarcinoma, it is impossible to determine whether the adenocarcinoma is primary or metastatic simply from HE sections (A). Positive staining of TTF-1 (C) and the absence of a transcript for MGB1 (D) suggests that this lung tumor is a primary pulmonary adenocarcinoma. By contrast, the metastatic cancer in the lymph node specimen is negative for TTF-1 and positive for MGB1 (D), suggesting metastatic breast cancer. The Journal of Molecular Diagnostics 2004 6, 90-95DOI: (10.1016/S1525-1578(10)60495-3) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions