MODELING TEAM REPORT July 28, 2009 Mary & Leon

CONTENTS 1 Modeling Plan for SiGEM 2 Further Analysis on the IDO Enzyme Model 2 Mutated and Regular Trp-Repressor 3 The Production of B-carotene with IPP 4 BLH Enzyme Kinetics 5

1. Modeling Plan for SiGEM

Modeling Team Subprojects BLH Ming Trp-R Mark Anusuya NO-SoxS Suzie Crt Bobby IL-6 Export Robert IDO Ariana Joe

2. Further Analysis on the IDO Enzyme Model

The Reaction: 5MT--|Trp REACTION 1：L-tryptophan + O2 = N-formyl-L-kynurenine E REACTION 2: 5-methyl-tryptophan + O2 =4-(2-amino-5-methylphenyl)-2-(formylamino)-4-oxobutanoic acid

Observations

Observations

Observations

An Explanation with Equations

Speeding Up the Reaction [S]0 = 10mM [S]0 = 2.28e-6mM

3. Mutated and Regular Trp-Repressor

The Reaction: Trp/5MTDNA-P/O Input Output

Equations

Observation: [Trp]=0~0.01M

Observation: [Trp]=0~0.001M

The Reaction: 5MTIL-6 Output Input

Observation: 5MT->IL-6 Level of il-6 over time IL-6 Export?

4. The Production of B-carotene with IPP

The Reaction: 8*IPP->B-Carotene Output Input

5. BLH Enzyme Kinetics

The Reaction: B-CaroteneRetinoic Acid Output Input

Observations B-carotene input? BLH levels?

Thanks for your attention^^