TCR signaling induces nuclear actin polymerization.

Slides:

Advertisements

Similar presentations

High-dimensional analysis of lymphoid CD4+ T cells identified distinct TFH cell subsets in HIV+ patients and HCs. High-dimensional analysis of lymphoid.

Advertisements

Correlation between endogenous STAT5ab signaling and circulating plasma factors. Correlation between endogenous STAT5ab signaling and circulating plasma.

Immunologic responses after the MN-mediated cancer immunotherapy.

CD169+ macrophages play a critical role in mediating innate immune cell reorganization. CD169+ macrophages play a critical role in mediating innate immune.

Nuclear calcium signaling drives nuclear actin polymerization in T cells. Nuclear calcium signaling drives nuclear actin polymerization in T cells. (A)

Nuclear Arp3 mediates formation of TCR-induced nuclear actin filaments

Increased movement of GPR55 KO cells in association with epithelial cells. Increased movement of GPR55 KO cells in association with epithelial cells. (A)

Regulatory CD4+ T cell–derived IL-10 is important for B cell differentiation and the GC response. Regulatory CD4+ T cell–derived IL-10 is important for.

CD169+ macrophages mediate Lm translocation to the splenic T cell zones. CD169+ macrophages mediate Lm translocation to the splenic T cell zones. (A) Confocal.

Examples of AEGIS autonomous target selection.

Comparative analysis of the nuclear F-actin networks in A3

Altered distribution of γδT IELs in GPR55-deficient mice.

Human PBMC-derived MERS-CoV–specific T cells are multifunctional.

Cytosolic entry of Lm required for CD8α+ DC recruitment.

Arp2/3-mediated formation of nuclear actin networks is essential for CD4+ T cell effector functions. Arp2/3-mediated formation of nuclear actin networks.

Protection from autoimmunity is not due to the expansion of Treg subsets. Protection from autoimmunity is not due to the expansion of Treg subsets. (A.

VH usage of cross-reactive B cells induced by H5N1 or H7N9 vaccination

Reduced FOXO1 expression in GC B cells from mice lacking regulatory CD4+ T cell–derived IL-10. Reduced FOXO1 expression in GC B cells from mice lacking.

Tukey boxplots overlaid on data points from objective and subjective measures, displaying results from study 1. Tukey boxplots overlaid on data points.

Characterization of the light-responsive transdermal MNs.

Direct visualization by RNA FISH of TLR7 escape from X inactivation in B cells from women. Direct visualization by RNA FISH of TLR7 escape from X inactivation.

Donor and recipient BAL T cells are phenotypically and functionally memory T cells. Donor and recipient BAL T cells are phenotypically and functionally.

Differential expression of TRM markers by donor- and recipient-derived T cells with time. Differential expression of TRM markers by donor- and recipient-derived.

TFEB controls actin cytoskeleton reorganization in response to microbial sensing. TFEB controls actin cytoskeleton reorganization in response to microbial.

Murine gingival MSCs and skin MSCs produce and secrete IL-1RA–EV

Comparison of ternary TCR-lipid-CD1 complex structures.

Immune cell recruitment after the NIR-boosted and MN-mediated cancer immunotherapy. Immune cell recruitment after the NIR-boosted and MN-mediated cancer.

NCMs regulate T cell survival in TLOs via PD-L1.

PD-L1 expression is maintained on NCMs under inflammatory conditions and PD-L1+NCMs are found in TLOs. PD-L1 expression is maintained on NCMs under inflammatory.

Fig. 1 ZIKV RNA in blood and tissues.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

CD169+ macrophages mediate the transport of bacteria to T cell zones by trans-infecting CD8α+ DCs. CD169+ macrophages mediate the transport of bacteria.

Evaluation of multidonor class Ig sequences obtained from H5N1 and H7N9 vaccinated donors. Evaluation of multidonor class Ig sequences obtained from H5N1.

Microrobots with different cell-carrying capacities under different grid lengths (lg) and burr lengths (lb). Microrobots with different cell-carrying capacities.

Fig. 4 Rotation of nuclei in oocytes in the primordial follicles.

CD4+CLA+CD103+ T cells in skin and blood are clonally related.

The wild-type BRCA1-BARD1 EM structure resembles a clamp-like motif

Optimization of a MYC degradation screen.

Shared phenotype of CD4+CLA+CD103+ T cells from human blood and skin.

A LAT-dependent amino acid uptake system operates in both osteoclasts and osteoblasts. A LAT-dependent amino acid uptake system operates in both osteoclasts.

MYC degradation screen identifies a compound that stabilizes MYC protein. MYC degradation screen identifies a compound that stabilizes MYC protein. (A)

CD25 expression identifies two transcriptionally distinct subsets of very early effector cells. CD25 expression identifies two transcriptionally distinct.

Memory-biased TCRs induce weaker TCR signals than effector-biased TCRs in vitro. Memory-biased TCRs induce weaker TCR signals than effector-biased TCRs.

CD25 surface expression and TCR signal strength predict T helper differentiation and memory potential of early effector T cells in vivo. CD25 surface expression.

BMS blocks functional responses in primary immune cells driven by IFNα

Fig. 1 Bioinspired design of AAD for promoting wound contraction.

S-Affs colocalize with SUMO in mammalian cells.

Fig. 6 WPS imaging of different chemical components in living cells.

Galloping-like gait with the design of a two-legged robot.

Examples of AEGIS autonomous target selection.

Fig. 3 ET dynamics on the control and treatment watersheds during the pretreatment and treatment periods. ET dynamics on the control and treatment watersheds.

Fig. 1. Matrix stiffness sensitizes myofibroblasts to apoptosis induced by inhibition of their mechanotransduction pathways. Matrix stiffness sensitizes.

Calcium release through TRPML1 controls myosin IIA retrograde flow at the rear of LPS-DCs. Calcium release through TRPML1 controls myosin IIA retrograde.

Fig. 2 IRF8 is expressed in CD68+ macrophages after SCI.

GSDMD localizes to the plasma membrane and is processed during NET formation. GSDMD localizes to the plasma membrane and is processed during NET formation.

Fig. 1 Deficient CD73 and CD39 expressions and extracellular adenosine concentration in BM of OVX animals. Deficient CD73 and CD39 expressions and extracellular.

Initial testing and characterization of cAMPr in ES cells.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

IFN treatment of human midgestation villous explants induces syncytial knot formation. IFN treatment of human midgestation villous explants induces syncytial.

Fig. 5 Simulation of the mechanical properties of the 3DGraphene foam in a wide temperature range down to the cryogenic region. Simulation of the mechanical.

Fig. 5 Binding and fusion of HIV Env particles to SPPMs.

IL-9–expressing TH cells are highly enriched in CCR4+/CCR8+ effector memory TH cells. IL-9–expressing THcells are highly enriched in CCR4+/CCR8+effector.

Fig. 2 Supraballs and films from binary SPs.

Fig. 5 Reconstitution of the dormant state using fetal ovaries.

Fig. 3 Neuroblast-induced morphological dynamics of reactive astrocytes involve the inhibition of Cdc42-regulated actin polymerization. Neuroblast-induced.

Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.

Fig. 1 Differential anterograde transport of CCR5 and TNF.

The neuropod cells. The neuropod cells. (Top left) Neuropod cells synapse with sensory neurons in the small intestine, as shown in a confocal microscopy.

Intratumoral spatial distribution of granulocytes and T cells.

Fig. 3 4n hybrids undergo bipolar and tripolar mitosis.

Presentation transcript:

TCR signaling induces nuclear actin polymerization. TCR signaling induces nuclear actin polymerization. (A) Top: Jurkat NCLA cells were placed on TCR-stimulatory coverslips and subjected to live-cell microscopy. Shown are still images at the indicated time after contact with the stimulatory surface (see also movie S1). Bottom: Single-cell analysis of actin dynamics over 10 min after activation. Individual cells were imaged as in (A), and the periods in which polymerized F-actin was detected in the nucleus (green) or at the PM (red) are indicated. p.a., post activation. (B) Primary human CD4+ T cell expressing nuc.lifeact interacting with SEB-pulsed Raji B cells. Top: Representative still images of live-cell movies. Inset: Boxed contact region (cont.) in high contrast (see also movie S2). Bottom: Single-cell analysis of actin dynamics over 50 min after contact with the Raji B cell. (C to E) Nuclear actin polymerization in Jurkat NLA cells in response to surface-bound (C, top, and D) or soluble (C, bottom, and E) stimuli. Shown are representative images (see also movie S3) and relative occurrence of nuclear F-actin filaments (mean and SD from three experiments in which at least 30 cells were analyzed per condition each). Statistical significance relative to the αCD3/28 (D) or PMA/Iono (E) control was assessed by one-sample t test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. DMSO, dimethyl sulfoxide. Visualization of nuclear F-actin with nuclear lifeact.GFP after (+) or without (−) stimulation with PMA/Iono in primary human CD4+ T cells (F). Visualization of endogenous nuclear actin filaments with Alexa Fluor 488 Phalloidin in A3.01 T cells without stimulation (−) or after stimulation (+) with PMA/Iono (G). Scale bars, 5 μm. Arrowheads indicate examples of nuclear F-actin filaments. N. Tsopoulidis et al. Sci. Immunol. 2019;4:eaav1987 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works