Table 2. Set of reference peptide for retention time correction

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Table 2. Set of reference peptide for retention time correction Peptide Sequence HF* HF SSAAPPPPPR 7.6 MGVPYAIIK 30.6 GISNEGQNASIK 15.5 GILFVGSGVSGGEEGAR 34.5 HVLTSIGEK 15.6 SFANQPLEVVYSK 35.0 DIPVPKPK 17.7 IADISLAAFGR 35.8 NAGGVGELEK 18.3 LTILEELR 37.3 IGDYAGIK 19.2 EYVPSVIEPSFGIGR 40.1 GISSSAIPYSR 23.2 NGFILDGFPR 40.4 DLETLTMHTK 23.5 ELASGLSFPVGFK 41.2 YYTITEVATR 25.4 LSSEAPALFQFDLK 46.7 ELGQSGVDTYLQTK 28.4 *HF refers to the hydrophobicity factor calculated using the SSRCalc algorithm [1, 2]. [1] Krokhin, O. V., Sequence-specific retention calculator. Algorithm for peptide retention prediction in ion-pair RP-HPLC: application to 300- and 100-A pore size C18 sorbents. Anal Chem 78 7785-7795 (2006). [2] Spicer, V., Yamchuk, A., Cortens, J., Sousa, S., et al., Sequence-specific retention calculator. A family of peptide retention time prediction algorithms in reversed-phase HPLC: applicability to various chromatographic conditions and columns. Anal Chem 79 8762-8768 (2007).