Growth differentiation factor 9 concentration in the follicular fluid of infertile women with endometriosis Hendy Hendarto, Ph.D., Prajitno Prabowo,

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Growth differentiation factor 9 concentration in the follicular fluid of infertile women with endometriosis Hendy Hendarto, Ph.D., Prajitno Prabowo, M.D., Farid Anfasa Moeloek, Ph.D., Soetjipto Soetjipto, Ph.D. Fertility and Sterility Volume 94, Issue 2, Pages 758-760 (July 2010) DOI: 10.1016/j.fertnstert.2009.10.011 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Detection of protein with an approximate molecular weight of 53 kDa (red arrow) in extracts from follicular fluid using anti–growth differentiation factor 9 (GDF-9) antibodies. The weakest color intensity is shown in lanes 2 and 3, the strongest in lanes 6 and 7. M = mild endometriosis; N = no endometriosis; S = severe endometriosis. (B) Deglycosylated (dG) GDF-9 using PNGase F. This treatment reduces the size of 53 kDa band to 48 kDa. (C) Concentration (pg/mL) of GDF-9 in the follicular fluid by ELISA. Fertility and Sterility 2010 94, 758-760DOI: (10.1016/j.fertnstert.2009.10.011) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions