Comparison of the original ITS primer pair and the new, modified ITS primer pair. Comparison of the original ITS primer pair and the new, modified ITS.

Slides:

Advertisements

Similar presentations

Taxonomic composition of subway microbial communities.

Advertisements

Microbial community dissimilarity.

Fig. 1. The effect of dietary micronutrient deficiency on the configuration of a defined human gut microbiota established in gnotobiotic mice. The effect.

Relative abundances of the top 10 most abundant OTUs across all fish samples are shown along with the data from the probiotic B. pumilus RI06-95 (OTU 3673)

Heatmap summarizing the significant (P < 0

Microbial diversity was broadly compared across three unique built-environment study sites for 16S rRNA amplicon and shotgun metagenome sequencing. Microbial.

Transdomain taxonomic profiles from subway shotgun metagenomes.

Fractions of 16S rRNA genes from bacteria (top panel) and archaea (bottom panel) in public databases from primer-amplified metagenomes (with and without.

Bacterial biomarkers associated with disease severity.

Gut microbial diversity of mice in voluntary and forced exercise groups. Gut microbial diversity of mice in voluntary and forced exercise groups. Weighted.

Microbial diversity of the 10 body locations sampled.

Fluoride affects stool-derived taxa found in the oral cavity.

Putative MBTA microbial community sources.

Temporal and spatial patterns.

Skin Microbiome Surveys Are Strongly Influenced by Experimental Design

Taxonomic composition of the baboon and human gut microbiota.

Nonmetric multidimensional scaling (NMDS) plots of fecal and biopsy sample-based Bray-Curtis distances computed from the relative abundances of the 258.

Transcription levels of metabolic pathways and genes in mother and infant pair 4 at time point 2. Transcription levels of metabolic pathways and genes.

Bacteroidetes bacteria are the main contributors to LPS biosynthesis in the human gut microbiome. Bacteroidetes bacteria are the main contributors to LPS.

(A) Heat maps illustrate the percent abundances of the most abundant genera (all OTUs taxonomically classified to the same genus were collapsed into a.

Differences in microbiome between control and BPA-exposed rabbit offspring and dams according to linear discriminant analysis effect size (LEfSe). Differences.

Distribution of the bacterial taxa that exhibit the greatest log fold changes in mean relative abundance. Distribution of the bacterial taxa that exhibit.

Null modeling approach for quantifying influences of assembly processes and connecting those processes to biogeochemical function. Null modeling approach.

Prevalence and geographical distribution of Streptococcus equi in horse farms sampled in Israel during November–December Prevalence and geographical.

Population characteristics.

Unweighted UniFrac PCoA plots.

Differential relative abundance of major taxa for successive pairwise comparisons. Differential relative abundance of major taxa for successive pairwise.

The similar shifts of gut microbiota in IBD across cohorts.

Enterotypes of the distal gut microbial profiles.

Distribution of microbial functions in urban waterways.

Comparison of the modified 515f/806rB V4 primer pair and the 515f (modified)/926r primer pair. Comparison of the modified 515f/806rB V4 primer pair and.

Stability of fecal microbiomes in different preservatives and under different temperature treatments. Stability of fecal microbiomes in different preservatives.

Relative abundances of Akkermansia muciniphila organisms in all mouse groups. Relative abundances of Akkermansia muciniphila organisms in all mouse groups.

Proportion of 16S rRNA gene sequences in each category of phylogenetic novelty relative to cultures for each environment, by amplicons, metagenomes (without.

Field community comparisons via 16S and between sequencing runs.

Effects of FMT in sows and/or offspring on median relative abundances (%) of bacterial phyla (A and B) and genera (C and D) in offspring fecal samples.

Relative ITS2 abundance of Saccharomyces in stool over time as a Saccharomyces cerevisiae-free diet was consumed. Relative ITS2 abundance of Saccharomyces.

Metabolite predictability is consistent between vaginal and mouse cecal data sets. Metabolite predictability is consistent between vaginal and mouse cecal.

Label frequency and average enrichment of taxa.

(A) Box-and-whisker plots illustrate the median, maximum, minimum, and first and third quartiles of the distribution of the number of observed OTUs and.

Altered mycobiota and bacterial-fungal correlation in AS patients receiving different therapeutic regimens. Altered mycobiota and bacterial-fungal correlation.

Gut microbiota components influenced by AXOS intake.

Taxonomy assessment of metagenes with differential abundance as a result of the AXOS intervention. Taxonomy assessment of metagenes with differential abundance.

Microbial composition of mother and infant samples and shared bacteria within mother-infant pairs. Microbial composition of mother and infant samples and.

Kinetics of clone appearance, size, persistence, and lineage content.

Fig. 2 Ro60 commensal bacteria are common among lupus and healthy subjects without overt dysbiosis of the fecal, oral, or skin microbiome. Ro60 commensal.

Relative abundance (RA) of individual phyla that are strongly correlated (|rs| = >0.6; q =

Shifts in the microbial community compositions of the control and antibiotic groups. Shifts in the microbial community compositions of the control and.

(A and B) Unweighted (A) and weighted (B) UniFrac clustering of three 16S rRNA hypervariable regions using fragment insertion SATé-enabled phylogenetic.

Diversity of whale blow and seawater samples from minimum entropy decomposition (MED) node groupings (17), including observed number of MEDs, a relative.

Cyanophage-host interactions from metatranscriptomic data.

mtDNA genotypes correlate with gut microbiota composition.

A principal-coordinate analysis plot of UniFrac distances from Deblur as visualized by Emperor. A principal-coordinate analysis plot of UniFrac distances.

The ND6P25L mtDNA mutation reduces gut microbiota diversity in C57BL/6J mice. The ND6P25L mtDNA mutation reduces gut microbiota diversity in C57BL/6J mice.

Fig. 1 A phylogenetically cohesive core rumen microbiome was found across farms with highly conserved hierarchical structure and tight association to overall.

Plot of relative abundance of Bifidobacterium animalis (A), Ruminococcus (Ruminococcaceae) (B), and Succinivibrio (Succinivibrionaceae) (C) (repeated-measures.

Comparison of the original 515f/806r primer pair and the new, modified 515f/806rB primer pair. Comparison of the original 515f/806r primer pair and the.

PCoA plots of subgingival samples with disease classification overlaid

Shannon rarefaction analysis.

Relationships between antimicrobial chemicals, features of the built environment, and microbial communities. Relationships between antimicrobial chemicals,

Hierarchically clustered heat map showing correlations between the relative abundances of microbial species and the levels of volatile compounds in kefir.

Relative proportions of taxa and UPGMA hierarchical clustering of the mock communities. Relative proportions of taxa and UPGMA hierarchical clustering.

Acarbose reversibly changes the structure of the murine gut community.

Variations in beta and alpha diversity of gut microbiome bacterial communities in relation to presence of Blastocystis. Variations in beta and alpha diversity.

Variations in beta and alpha diversity of gut microbiome eukaryotic communities explained by presence of Blastocystis. Variations in beta and alpha diversity.

Integrated analysis of gene expression and copy number alterations.

Effect of bloom filtering on American Gut data.

Comparison of 16S sequencing and shallow shotgun recovery of species-level taxa. Comparison of 16S sequencing and shallow shotgun recovery of species-level.

Infant skin and oral mycobiomes by birth mode.

Two to 20 years: female patients, continued.

Presentation transcript:

Comparison of the original ITS primer pair and the new, modified ITS primer pair. Comparison of the original ITS primer pair and the new, modified ITS primer pair. (A) Procrustes plot of original and modified ITS1 constructs, Bray-Curtis dissimilarity M2 = 0.363. (B) Pie charts illustrating the mean relative abundance of phyla present (all studies combined) in samples amplified with the old ITS construct and the modified ITS construct. (C) Taxa scatterplots for the original and modified ITS primers. Phyla plots are shown for American Gut fecal, American Gut skin, agricultural soils, EMP Rice Rhizome, Body Farm 1, Body Farm 2, mouse decomposition, and Sloan built environment samples. Outlier samples have been removed from the data shown. William Walters et al. mSystems 2016; doi:10.1128/mSystems.00009-15