Vitrification is not superior to rapid freezing of normozoospermic spermatozoa: effects on sperm parameters, DNA fragmentation and hyaluronan binding

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Presentation transcript:

Vitrification is not superior to rapid freezing of normozoospermic spermatozoa: effects on sperm parameters, DNA fragmentation and hyaluronan binding Azam Agha-Rahimi, Mohammad Ali Khalili, Ali Nabi, Sareh Ashourzadeh Reproductive BioMedicine Online Volume 28, Issue 3, Pages 352-358 (March 2014) DOI: 10.1016/j.rbmo.2013.11.015 Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Comparison of sperm parameters after 24h for vitrification and rapid freezing methods. All parameters were significantly lower for rapid freezing and vitrification compared with fresh samples (P<0.0001). Values are mean and standard deviation. Reproductive BioMedicine Online 2014 28, 352-358DOI: (10.1016/j.rbmo.2013.11.015) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 (A) Classification of abnormal morphology in fresh, rapid freezing and vitrification groups; sperm tail and midpiece defects increased after cryopreservation (P<0.0001); values are mean and standard deviation. (B) Photomicrograph of frozen–thawed spermatozoa showing tail abnormalities including coiled (thin arrow) and bent (bold arrow); bar=10μm. Reproductive BioMedicine Online 2014 28, 352-358DOI: (10.1016/j.rbmo.2013.11.015) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Pearson correlation between total motility and viability (A) and morphology (B) in vitrification group. Reproductive BioMedicine Online 2014 28, 352-358DOI: (10.1016/j.rbmo.2013.11.015) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions