Invariant NKT Cells Suppress CD8+ T-Cell–Mediated Allergic Contact Dermatitis Independently of Regulatory CD4+ T Cells Anne Goubier, Marc Vocanson, Claire.

Slides:

Advertisements

Similar presentations

Cheng-Ming Sun, Edith Deriaud, Claude Leclerc, Richard Lo-Man Immunity

Advertisements

Nonpathogenic Bacteria Alleviating Atopic Dermatitis Inflammation Induce IL-10- Producing Dendritic Cells and Regulatory Tr1 Cells Thomas Volz, Yuliya.

Therapeutic Potential of B and T Lymphocyte Attenuator Expressed on CD8+ T Cells for Contact Hypersensitivity Daiki Nakagomi, Kotaro Suzuki, Junichi.

Volume 137, Issue 3, Pages (September 2009)

Identification of CD3+CD4−CD8− T Cells as Potential Regulatory Cells in an Experimental Murine Model of Graft-Versus-Host Skin Disease (GVHD) Fumi Miyagawa,

Tatsukuni Ohno, Yuta Kondo, Chenyang Zhang, Siwen Kang, Miyuki Azuma

Volume 35, Issue 4, Pages (October 2011)

CD90+ Human Dermal Stromal Cells Are Potent Inducers of FoxP3+ Regulatory T Cells Karin Pfisterer, Karoline M. Lipnik, Erhard Hofer, Adelheid Elbe-Bürger

Dissolving Microneedle Delivery of Nanoparticle-Encapsulated Antigen Elicits Efficient Cross-Priming and Th1 Immune Responses by Murine Langerhans Cells

Epicutaneous and Oral Low-Zone Tolerance Protects from Colitis in Mice

Crosstalk of regulatory T cells and tolerogenic dendritic cells prevents contact allergy in subjects with low zone tolerance Ulrike Luckey, PhD, Talkea.

Histamine receptor H1 signaling on dendritic cells plays a key role in the IFN-γ/IL-17 balance in T cell–mediated skin inflammation Béatrice Vanbervliet,

Langerin+ Dermal DC, but Not Langerhans Cells, Are Required for Effective CD8- Mediated Immune Responses after Skin Scarification with Vaccinia Virus

Down-Regulation of CD62L Shedding in T Cells by CD39+ Regulatory T Cells Leads to Defective Sensitization in Contact Hypersensitivity Reactions Karsten.

Absence of CCR4 Exacerbates Skin Inflammation in an Oxazolone-Induced Contact Hypersensitivity Model Sari Lehtimäki, Sari Tillander, Anne Puustinen,

Γδ T Cells Augment Rejection of Skin Grafts by Enhancing Cross-Priming of CD8 T Cells to Skin-Derived Antigen Azad Rahimpour, Stephen R. Mattarollo,

Α-MSH-Stimulated Tolerogenic Dendritic Cells Induce Functional Regulatory T Cells and Ameliorate Ongoing Skin Inflammation Matteo Auriemma, Thomas Brzoska,

Volume 137, Issue 3, Pages (September 2009)

Volume 18, Issue 5, Pages (May 2003)

Inhibition of Cathepsin S Reduces Allogeneic T Cell Priming but Not Graft-versus-Host Disease Against Minor Histocompatibility Antigens Hisaki Fujii,

Volume 140, Issue 7, Pages (June 2011)

The Aryl Hydrocarbon Receptor Is Involved in UVR-Induced Immunosuppression Fatemeh Navid, Anika Bruhs, Winfried Schuller, Ellen Fritsche, Jean Krutmann,

Langerhans Cells Are Required for UVR-Induced Immunosuppression

Plasmacytoid Dendritic Cells Mediate Oral Tolerance

NKT Cells Inhibit the Onset of Diabetes by Impairing the Development of Pathogenic T Cells Specific for Pancreatic β Cells Lucie Beaudoin, Véronique.

IL-10-Producing Langerhans Cells and Regulatory T Cells Are Responsible for Depressed Contact Hypersensitivity in Grafted Skin Ryutaro Yoshiki, Kenji.

Natural Killer T Cells Are Essential for the Development of Contact Hypersensitivity in BALB/c Mice Chihiro Shimizuhira, Atsushi Otsuka, Tetsuya Honda,

Capsiate Inhibits DNFB-Induced Atopic Dermatitis in NC/Nga Mice through Mast Cell and CD4+ T-Cell Inactivation Ji H. Lee, Yun S. Lee, Eun-Jung Lee, Ji.

Toll-Like Receptor 3 Increases Allergic and Irritant Contact Dermatitis Naomi Nakamura, Risa Tamagawa-Mineoka, Mayumi Ueta, Shigeru Kinoshita, Norito.

Urupongsa Ausaneya, Akira Kawada, Yoshinori Aragane

Jin-Sung Chung, Kyoichi Tamura, Ponciano D. Cruz, Kiyoshi Ariizumi

Mast Cells Play a Key Role in Host Defense against Herpes Simplex Virus Infection through TNF-α and IL-6 Production Rui Aoki, Tatsuyoshi Kawamura, Fumi.

Activation of the Arylhydrocarbon Receptor Causes Immunosuppression Primarily by Modulating Dendritic Cells Anika Bruhs, Thomas Haarmann-Stemmann, Katrin.

Integrin αE(CD103) Is Involved in Regulatory T-Cell Function in Allergic Contact Hypersensitivity Andrea Braun, Nadin Dewert, Fiona Brunnert, Viktor.

Volume 14, Issue 1, Pages (January 2001)

Georg Varga, Nadine Nippe, Sandra Balkow, Thorsten Peters, Martin K

CD4+ T Cells in Lymph Nodes of UVB-Irradiated Mice Suppress Immune Responses to New Antigens Both In Vitro and In Vivo Shelley Gorman, Jamie W.-Y. Tan,

Topical Imiquimod Treatment Prevents UV-Light Induced Loss of Contact Hypersensitivity and Immune Tolerance Thomas H. Thatcher, Irina Luzina, Rita Fishelevich,

Volume 24, Issue 2, Pages (February 2006)

The Differential Role of L-Selectin and ICAM-1 in Th1-Type and Th2-Type Contact Hypersensitivity Asako Ogawa, Ayumi Yoshizaki, Koichi Yanaba, Fumihide.

Lymphatic Dysfunction Impairs Antigen-Specific Immunization, but Augments Tissue Swelling Following Contact with Allergens Makoto Sugaya, Yoshihiro Kuwano,

Impaired Initiation of Contact Hypersensitivity by FTY720

Indoleamine 2,3-Dioxygenase Activity Contributes to Local Immune Suppression in the Skin Expressing Human Papillomavirus Oncoprotein E7 Deepak Mittal,

The Temporal and Spatial Dynamics of Foxp3+ Treg Cell–Mediated Suppression during Contact Hypersensitivity Responses in a Murine Model Sari Lehtimäki,

Shelley Gorman, Melinda A. Judge, Prue H. Hart

Β-Arrestin 2 Inhibits Proinflammatory Chemokine Production and Attenuates Contact Allergic Inflammation in the Skin Evelyn Gaffal, Mira Jakobs, Nicole.

Contact Hypersensitivity in MHC Class II-Deficient Mice Depends on CD8 T Lymphocytes Primed by Immunostimulating Langerhans Cells Anne Bouloc, Andrea.

Afferent and Efferent Phases of Allergic Contact Dermatitis (ACD) Can Be Induced After a Single Skin Contact with Haptens: Evidence Using a Mouse Model.

CD8+ Cytotoxic T Cells Induce Relapsing Colitis in Normal Mice

Jeffery M. Cowden, Mai Zhang, Paul J. Dunford, Robin L. Thurmond

Volume 135, Issue 3, Pages (September 2008)

Volume 34, Issue 3, Pages (March 2011)

Timothy J. Paradis, Susan H. Cole, Robin T. Nelson, Ronald P. Gladue

1,25-Dihydroxyvitamin D Exerts Similar Immunosuppressive Effects as UVR but Is Dispensable for Local UVR-Induced Immunosuppression Agatha Schwarz, Fatemeh.

Agatha Schwarz, Anika Bruhs, Thomas Schwarz

Regulatory T Cells from IL-10-Deficient Mice Fail to Suppress Contact Hypersensitivity Reactions Due to Lack of Adenosine Production Sabine Ring, Alexander.

Urocanic Acid: An Endogenous Regulator of Langerhans Cells

In Vivo Expansion of Regulatory T cells With IL-2/IL-2 mAb Complexes Prevents Anti- factor VIII Immune Responses in Hemophilia A Mice Treated With Factor.

Priming of Leishmania-Reactive CD8+ T cells In Vivo Does Not Require LMP7- Containing Immunoproteasomes Sven Brosch, Stefan Tenzer, Nadja Akkad, Beate.

Deficient Contact Hypersensitivity Reaction in CD4−/− Mice Is Because of Impaired Hapten-Specific CD8+ T Cell Functions Pierre Saint-Mezard, Cyril Chavagnac,

IL-23 Antagonizes UVR-Induced Immunosuppression through Two Mechanisms: Reduction of UVR-Induced DNA Damage and Inhibition of UVR-Induced Regulatory T.

Antigen-Specific Suppression of a Primed Immune Response by Dendritic Cells Mediated by Regulatory T Cells Secreting Interleukin-10 Ela Martin, Brendan.

Basophil-Derived Amphiregulin Is Essential for UVB Irradiation–Induced Immune Suppression Chantal Meulenbroeks, Huib van Weelden, Christian Schwartz,

Interleukin-21 Inhibits Dendritic Cell-Mediated T Cell Activation and Induction of Contact Hypersensitivity In Vivo Donald C. Foster, Ralf Paus Journal.

Volume 28, Issue 5, Pages (May 2008)

Cutaneous Hypersensitivities to Hapten Are Controlled by IFN-γ-Upregulated Keratinocyte Th1 Chemokines and IFN-γ-Downregulated Langerhans Cell Th2 Chemokines

SOCS1 Prevents Potentially Skin-Reactive Cytotoxic T Lymphocytes from Gaining the Ability to Cause Inflammatory Lesions Galaxia Maria Rodriguez, Dante.

CpG Immunostimulatory Sequences Enhance Contact Hypersensitivity Responses in Mice Hitoshi Akiba, Masataka Satoh, Keiji Iwatsuki, Dominique Kaiserlian,

Interleukin-10-Treated Dendritic Cells Modulate Immune Responses of Naive and Sensitized T Cells In Vivo Gabriele Müller, Anke Müller Journal of Investigative.

UV Exposure Boosts Transcutaneous Immunization and Improves Tumor Immunity: Cytotoxic T-Cell Priming through the Skin Pamela Stein, Gerd Rechtsteiner,

Presentation transcript:

Invariant NKT Cells Suppress CD8+ T-Cell–Mediated Allergic Contact Dermatitis Independently of Regulatory CD4+ T Cells Anne Goubier, Marc Vocanson, Claire Macari, Gaelle Poyet, André Herbelin, Jean- François Nicolas, Bertrand Dubois, Dominique Kaiserlian Journal of Investigative Dermatology Volume 133, Issue 4, Pages 980-987 (April 2013) DOI: 10.1038/jid.2012.404 Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Contact hypersensitivity (CHS) to DNFB is downregulated by invariant natural killer T (iNKT) cells. CHS to DNFB was tested in various settings. (a, b) CHS response of CD1d−/− (black circle) compared with CD1d+/+ littermates (a, white circle) or B6 (b, white circle) mice. (c, d) CHS response of Jα18−/− mice (black circle) compared with B6 (c, white circle) mice or Jα18−/− mice treated on days -1, 0, +1, and +4 with the anti-NK1.1 PK136 mAb (d, white circles). (e, f) CHS in B6 mice either untreated (white circle) or injected with PK136 (black circle) on (e) days -3, -2, and -1 before sensitization or on (f) days +4, +5, and +6 after sensitization. Results are expressed as the mean±SD ear swelling at different times after challenge, and are representative of two independent experiments using five to seven mice per group. *P<0.05, **P<0.01, Mann–Whitney U-test. Journal of Investigative Dermatology 2013 133, 980-987DOI: (10.1038/jid.2012.404) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Enhanced priming and skin recruitment of CD8 effectors in CD1d−/− and Jα18−/− mice. (a, b) Hapten-specific CD8+ T-cell response in lymph nodes (LNs) of day 5 DNFB-sensitized C57BL/6 (white bars), CD1d−/− (black bars), and Jα18−/− (hatched bars) mice. (a) Hapten-specific proliferation of CD8+ T cells (mean±SD of triplicate wells) measured by tritiated thymidine (3H-TdR) uptake. (b) Frequency of hapten-specific IFN-γ spot forming cells (SFCs) in draining LNs (dLNs) determined by ELISPOT (mean±SD SFC/106 CD8+ T cells of triplicate wells containing pooled CD8+ T cells from four mice per group). (a, b) Data are representative of one out of three experiments. (c) Number (No.) of CD8+ T cells in the ears of B6 (white bars) and CD1d−/− (black bars) mice at 48hours after DNFB challenge was determined by FACS analysis. The results show mean±SD obtained using three mice per group and are representative of one out of two experiments. c.p.m., counts per minute. Journal of Investigative Dermatology 2013 133, 980-987DOI: (10.1038/jid.2012.404) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Transfer of invariant natural killer T (iNKT) cells normalizes CD8+ T-cell and contact hypersensitivity (CHS) responses in Jα18−/− mice. (a, b) Frequency of hapten-specific IFN-γ spot forming cells (SFCs) in draining lymph nodes (dLNs) at (a) day 5 after sensitization or (b) day 2 after challenge in Jα18−/− mice that were either untransferred (white bars) or transferred (black bars) with purified liver naive iNKT cells 1 day before either (a) sensitization or (b) challenge with DNFB. Data correspond to mean±SD SFC/106 LN cells of triplicate wells containing pooled LN cells from four mice per group, and are representative of one out of two experiments. (c) CHS to DNFB in B6 (circles) and Jα18−/− (triangles) mice either untransferred (white symbols) or transferred 1 day before challenge (black symbols) with 4 × 106 liver B6 leukocytes. Each symbol represents the ear swelling of individual mouse at 48hours after challenge and horizontal bars indicate the mean±SD of 5 mice per group. Data are representative of one out of two experiments. *P<0.05, Mann–Whitney test. NS, nonsignificant. Journal of Investigative Dermatology 2013 133, 980-987DOI: (10.1038/jid.2012.404) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Invariant natural killer T (iNKT) cells control the contact hypersensitivity (CHS) response independently of CD4+ regulatory T cells (Tregs). (a) Number of splenic CD4+, CD8+, and FoxP3+ T cells in B6, Jα18−/−, and CD1d−/− mice (mean±SEM, four mice per group). NS (nonsignificant), Mann–Whitney test. (b) CD4+FoxP3+inducible costimulatory molecule (ICOS)+Tregs in draining lymph nodes (dLNs) of day 5 sensitized B6, Jα18−/−, and CD1d−/− mice. Horizontal bars show mean percentages of four mice (NS, Mann–Whitney test). (c) In vivo proliferation of carboxyfluorescein diacetate succinimidyl ester (CFSE)+CD4+FoxP3+ Tregs from day 6 sensitized B6 mice, 3 days after transfer and sensitization in Jα18−/− recipients (black dots) alone or together with naive NKT cells. Gray dots represent endogenous cells. (d) Kinetics of post-challenged CD4+CD25+ICOS+ Tregs infiltration in the ears of sensitized B6 and CD1d−/− mice (mean±SEM, three mice per group). (e) Suppression of in vitro proliferation (measured by uptake of tritiated thymidine [3H-TdR]) of DNFB-specific CD8+ T cells (effector T-cell, Teff) by Tregs from DNFB-sensitized mice with or without naive NKT cells. (f) CHS in Jα18−/− (circles) and CD1d−/− (squares) mice treated (black symbols) or not (open symbols) with an anti-CD4 mAb. Each symbol represents the mean±SD of ear swelling values from five to six mice (*P<0.05, **P<0.01, Mann–Whitney test). c.p.m., counts per minute. Journal of Investigative Dermatology 2013 133, 980-987DOI: (10.1038/jid.2012.404) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 CD1d-deficient dendritic cells (DCs) generate exacerbated contact hypersensitivity (CHS) response to DNFB. CHS response in B6 (circles) and CD1d−/− (triangles) mice immunized with hapten-pulsed bone marrow–derived dendritic cells (BM-DCs) from B6 (white symbols) or CD1d−/− (black symbols) mice and DNFB challenged 5 days later. Each symbol corresponds to the ear swelling of individual mouse at 48hours after challenge and horizontal bars represent the mean±SD of 7–13 mice per group. **P<0.01, Mann–Whitney test. Data are representative of two independent experiments. NS, nonsignificant. Journal of Investigative Dermatology 2013 133, 980-987DOI: (10.1038/jid.2012.404) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Contact hypersensitivity (CHS) downregulation by invariant natural killer T (iNKT) cell–derived cytokines induced by IFN-γ from CD8+ effectors. (a–c) IL-4 and IL-13 production by liver iNKT cells was tested in cocultures with (a) 2,4-dinitrobenzene-sulfonic acid (DNBS)-pulsed bone marrow–derived dendritic cells (BM-DCs) from B6 (black bars) or CD1d−/− (white bars) mice with or without CD8+ effector T cells (CD8eff) and anti-CD1d mAb; (b) DNBS-pulsed BM-DCs and CD8eff from either B6 or IL-4/IL-13−/− (4/13−/−) mice; and (c) hapten-pulsed BM-DCs with naive (CD8N) with or without exogenous IFN-γ or effector (CD8eff) CD8+ T cells; wt, wild type. Cytokine released in 72-hour supernatant (pool of triplicate wells) are representative of one out of three experiments. (d) Ear swelling in IL-4/IL-13−/− (IL4/13−/−; triangles) and IL-4/IL-13+/+ (IL4/13+/+; circles) mice at 24hours after DNFB challenge in sensitized (white symbols) or naive (black symbols) mice. Data of individual mice are shown. Horizontal bars indicate mean±SD of values pooled from three experiments. **P<0.01, ****P<0.0001, Mann–Whitney U-test. NS, nonsignificant. Journal of Investigative Dermatology 2013 133, 980-987DOI: (10.1038/jid.2012.404) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions