Edouard I. Azzam, Hatsumi Nagasawa, Yongjia Yu, Chuan-Yuan Li, John B

Slides:

Advertisements

Similar presentations

Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,

Advertisements

Peroxiredoxin is Ubiquitously Expressed in Rat Skin: Isotype-Specific Expression in the Epidermis and Hair Follicle Jeong Eun Lee, Byung Dae Kwon, Jee-Bum.

Tumor Necrosis Factor α Increases and α-Melanocyte-Stimulating Hormone Reduces Uveal Melanoma Invasion Through Fibronectin Irene Cantón, Paula C. Eves,

A New View of Vitiligo: Looking at Normal-Appearing Skin

Green Tea Extract and (−)-Epigallocatechin-3-Gallate Inhibit Mast Cell-Stimulated Type I Collagen Expression in Keloid Fibroblasts via Blocking PI-3K/Akt.

Evidence of Heterogeneity and Quantitative Differences of the Type 1 5α-Reductase Expression in Cultured Human Skin Cells – Evidence of its Presence in.

Jennifer L. Rizzo, Jessica Dunn, Adam Rees, Thomas M. Rünger

Effects of XPD Mutations on Ultraviolet-Induced Apoptosis in Relation to Skin Cancer- Proneness in Repair-Deficient Syndromes Sophie Queille, Christiane.

Ultraviolet Modulation of Human Macrophage Metalloelastase in Human Skin In Vivo Jin Ho Chung, Jin Young Seo, Mi Kyoung Lee, Hee Chul Eun, Joo Heung Lee,

Intense pulsed light irradiation may reverse activator protein-1 expression induced by ultraviolet B light Gwo-Shing Chen, MD, PhD, Hui-Chuan Lin, MS,

Assembly of Epithelial Cell Fibrillins

An Actin-Binding Protein Espin Is a Growth Regulator for Melanoma

Clinical, Cellular, and Molecular Features of an Israeli Xeroderma Pigmentosum Family with a Frameshift Mutation in the XPC Gene: Sun Protection Prolongs.

High Frequency of Loss of Heterozygosity on Chromosome Region 9p21–p22 but Lack of p16INK4a/p19ARF Mutations in Greek Patients with Basal Cell Carcinoma.

Einar K. Rofstad, Bjørn A. Graff Journal of Investigative Dermatology

Neurofibromatosis Type 1 Protein and Amyloid Precursor Protein Interact in Normal Human Melanocytes and Colocalize with Melanosomes Sofie De Schepper,

Protein Phosphatase Type 1-Dependent Dephosphorylation of the Retinoblastoma Tumor Suppressor Protein in Ultraviolet-Irradiated Human Skin and Keratinocytes

Elise S. Bales, Cheryl Dietrich, Debdutta Bandyopadhyay, Denise J

Histone Deacetylase 2 Is Upregulated in Normal and Keloid Scars

Sri Rajalakshmi Rudrabhatla, Christie L. Mahaffey, Mark E. Mummert

Monika Jost, Csaba Kari, Ulrich Rodeck

Adiam W. Bahta, Nilofer Farjo, Bessam Farjo, Mike P. Philpott

Expression of Matrilin-2 in Human Skin

UV Induces p21WAF1/CIP1 Protein in Keratinocytes Without p53

Daniel Hohl, Daniel Aeschlimann, Marcel Huber

Michaela Brenner, Sergio G. Coelho, Janusz Z. Beer, Sharon A

Mutant Loricrin is Not Crosslinked into the Cornified Cell Envelope but is Translocated into the Nucleus in Loricrin Keratoderma Akemi Ishida-Yamamoto,

Francois le Pelletier, Anne Janin Journal of Investigative Dermatology

A Newly Identified Patient with Clinical Xeroderma Pigmentosum Phenotype has a Non- Sense Mutation in the DDB2 Gene and Incomplete Repair in (6-4) Photoproducts

Compound Heterozygous TGM1 Mutations Including a Novel Missense Mutation L204Q in a Mild Form of Lamellar Ichthyosis Masashi Akiyama, Itsuro Matsuo

Defective Extracellular Matrix Reorganization by Chronic Wound Fibroblasts is Associated with Alterations in TIMP-1, TIMP-2, and MMP-2 Activity Helen.

Deletion of the Cytoplasmatic Domain of BP180/Collagen XVII Causes a Phenotype with Predominant Features of Epidermolysis Bullosa Simplex Marcel Huber,

Longwave UV Light Induces the Aging-Associated Progerin

Masahiro Hara, Mina Yaar, H

Clinical Snippets Journal of Investigative Dermatology

Minutes of the Board of Directors Meeting

Prevalence of Antibodies Against Virus-Like Particles of Epidermodysplasia Verruciformis-Associated HPV8 in Patients at Risk of Skin Cancer Sabine Stark,

UVB and Proinflammatory Cytokines Synergistically Activate TNF-α Production in Keratinocytes through Enhanced Gene Transcription Muhammad M. Bashir,

Loss of Normal Profilaggrin and Filaggrin in Flaky Tail (ft/ft) Mice: an Animal Model for the Filaggrin-Deficient Skin Disease Ichthyosis Vulgaris Richard.

Protease-Activated Receptor 2, a Receptor Involved in Melanosome Transfer, is Upregulated in Human Skin by Ultraviolet Irradiation Glynis Scott, Cristina.

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

High Frequency of Hermansky–Pudlak Syndrome Type 1 (HPS1) Among Japanese Albinism Patients and Functional Analysis of HPS1 Mutant Protein Shiro Ito,

Journal of Investigative Dermatology

Journal of Investigative Dermatology

MAP Kinase Abnormalities in Hyperproliferative Cultured Fibroblasts from Psoriatic Skin Stéphanie Dimon-Gadal, Françoise Raynaud, Danièle Evain-Brion,

Masaki Nagai, Minoru Hasegawa, Kazuhiko Takehara, Shinichi Sato

Society for Investigative Dermatology 2010 Meeting Minutes

BJD Editor's Choice Journal of Investigative Dermatology

Cells of Origin in Skin Cancer

Reduced Hyaluronan in Keloid Tissue and Cultured Keloid Fibroblasts

Research Snippets Journal of Investigative Dermatology

In Vitro Differences Between Keratinocyte Stem Cells and Transit-Amplifying Cells of the Human Hair Follicle Cecilia Roh, Qingfeng Tao, Christos Photopoulos,

Apolipoprotein E is Present in Primary Localized Cutaneous Amyloidosis

Research Snippets from the British Journal of Dermatology

Melanocyte Regeneration in Vitiligo Requires WNT beneath their Wings

TLR3: A Receptor that Recognizes Cell Injury Is Essential for Permeability Barrier Homeostasis Following UV Irradiation Kenneth R. Feingold Journal.

25 Years of Epidermal Stem Cell Research

Production of Low Titers of Anti-Desmoglein 1 IgG Autoantibodies in Some Patients with Staphylococcal Scalded Skin Syndrome Hidemi Anzai, John R. Stanley,

Research Snippets Journal of Investigative Dermatology

Journal of Investigative Dermatology

Immunoprotective UVA (320–400 nm) Irradiation Upregulates Heme Oxygenase-1 in the Dermis and Epidermis of Hairless Mouse Skin Munif Allanson, Vivienne.

Lack of Membrane Expression of Interleukin-2 Receptor α Chain (CD25) in Mycosis Fungoides: Application of Laser Scanning Cytometry for Phenotyping of.

Journal of Investigative Dermatology

Journal of Investigative Dermatology

CD40 Ligation Alters the Cell Cycle of Differentiating Keratinocytes

IL-12 Completely Blocks Ultraviolet-Induced Secretion of Tumor Necrosis Factor α from Cultured Skin Fibroblasts and Keratinocytes Victoria P. Werth,

Modulation of IL-10, IL-12, and IFN-γ in the Epidermis of Hairless Mice by UVA (320– 400 nm) and UVB (280–320 nm) Radiation Jie Shen, Shisan Bao, Vivienne.

Catherine Booth, Christopher S. Potten

Lung Adenocarcinoma Harboring Mutations in the ERBB2 Kinase Domain

Ulrike P. Kappes, Dan Luo, Marisa Potter, Karl Schulmeister, Thomas M

Presentation transcript:

Cell Cycle Deregulation and Xeroderma Pigmentosum Group C Cell Transformation Edouard I. Azzam, Hatsumi Nagasawa, Yongjia Yu, Chuan-Yuan Li, John B. Little, MD Journal of Investigative Dermatology Volume 119, Issue 6, Pages 1350-1354 (December 2002) DOI: 10.1046/j.1523-1747.2002.19628.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Expression of p53, p21Waf1, HDM2, and p34cdc2 3–48 h postirradiation in control (C) and γ-irradiated (5.4 Gy) GM2995 fibroblasts at various culture passages. AG1521 is a normal control fibroblast strain (p=passage number). Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 p53 and p21Waf1 expression 3–48 h postirradiation in control (C) and γ-irradiated (5.4 Gy) GM2996 cells at different culture passages (p=passage number). Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 p53 and p21Waf1 expression in control (C) and UVC-irradiated (V) GM2995 cells. (A) Expression 3 h after irradiation of cells at passage levels 9, 15, and 28. (B) Expression in cells at passage 9 and 28 examined 3, 6, or 48 h after irradiation. Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 ATM expression in various cell strains 3 h after exposure to γ-irradiation. T2, B9, and 1264 are skin fibroblast cell strains isolated from patients with the Li–Fraumeni syndrome. Shown is a slowly migrating protein of molecular weight 100–120 kDa that reacts with the anti-ATM antibody. The second (upper) band was seen consistently in high passage GM2995 cells. The high molecular weight ATM protein (350 kDa) observed in all cells at all passage levels is not shown. Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Single-strand conformational polymorphism analysis of exons 6–9 of the P53gene in GM 2995 (XPC5) cells at passages 8, 17, and 28, indicating a mutation in exon 8 had occurred by passage 17. AG1521 is a normal control cell strain. Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 (I) Cell morphology of colonies of GM2995 cells in monolayer culture after seeding single cells at low density. (A) GM2995 (passage 6); (B) GM2995 (passage 13); (C) GM2995 (passage 13) (dense colony). The cultures were fixed with methanol and stained with Giemsa for 10 min.(II) Appearance of colonies in soft agar. (A) GM2995 (passage 6); (B) GM2995 (passage 14); (C) GM2995 (passage 31). Bars=0.1 mm. Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 p16ink4and p27Kip1 expression in control (C) and γ-irradiated (5.4 Gy) GM2995 cells examined at passages 9 and 15. Expression levels at 3.5, 26, and 48 h after irradiation are shown. Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 8 Phosphorylation of pRb in control and γ-irradiated GM2995 cells examined 2–24 h postirradiation in cells at passage 7 or 15. Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 9 VHL expression in control (C) and UVC-irradiated (V) GM2995 cells examined at passages 9 or 28. Journal of Investigative Dermatology 2002 119, 1350-1354DOI: (10.1046/j.1523-1747.2002.19628.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions