Molecular Biotechnology

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Molecular Biotechnology 30-11-2018 1 2 3 4 5 * 1-HindIII 2-EcoRI 3 –BamHI 4 – PstI 5- Molecular weight marker * hybridization signal (weak, more clear in the membrane) Since there is no specific hybridization signal in all the wells of this membrane, use for your report the “reference membrane “ for the comparison with the in silico analysis. Do not forget to compare this result with the gel of the restriction of genomic DNA – try to explain why there is no signal in the other lanes