Jianbo Song, Danielle Mercer, Xiaofeng Hu, Henry Liu, Marilyn M. Li

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Common Leukemia- and Lymphoma-Associated Genetic Aberrations in Healthy Individuals Jianbo Song, Danielle Mercer, Xiaofeng Hu, Henry Liu, Marilyn M. Li The Journal of Molecular Diagnostics Volume 13, Issue 2, Pages 213-219 (March 2011) DOI: 10.1016/j.jmoldx.2010.10.009 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Transcription of MLL PTDs in peripheral blood samples from healthy individuals. Upper panel, MLL PTD transcription. Lanes 1 and 8 show e11/e3 fusion, and lane 6 shows e9/e3 fusion. Lower panel, Corresponding internal positive controls using primers for the abl gene. B, blank; M, marker. The Journal of Molecular Diagnostics 2011 13, 213-219DOI: (10.1016/j.jmoldx.2010.10.009) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 MLL PTDs in peripheral blood samples from healthy individuals at the genomic level. Seminested PCR using genomic DNA from individuals positive for MLL PTD transcripts. B, blank; positive control from a patient with AML. The Journal of Molecular Diagnostics 2011 13, 213-219DOI: (10.1016/j.jmoldx.2010.10.009) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Transcription of MLL PTDs in cord blood samples from healthy newborns. Upper panel, MLL PTD transcripts. Lower panel, ABL transcripts in the corresponding samples are shown as internal positive controls. M, marker; B, blank. The Journal of Molecular Diagnostics 2011 13, 213-219DOI: (10.1016/j.jmoldx.2010.10.009) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 4 Results of K562 cell dilution experiment. Cell line K562 was used to dilute cultured fibroblast cells. A: Results of primary PCR using A <–> B primer set for BCR-ABL p210. Dilution ratios are shown. B: Results of nested PCR using the internal C <–>D primer set. C: β-actin. F, cultured fibroblast cells; H2O, blank control; K-RT, K562 RNA control reverse transcription without adding reverse transcriptase (RT); M, 100-bp DNA ladder. The Journal of Molecular Diagnostics 2011 13, 213-219DOI: (10.1016/j.jmoldx.2010.10.009) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 5 Transcripts of PML-RARA in peripheral blood samples from healthy individuals. Lanes 1 to 4, healthy individuals. Individuals 2, 3, and 4 are positive for PML-RARA. M, marker; L form, long form, 427-bp PML-RARA chimeric transcript; S form, short form, 393-bp PML-RARA chimeric transcript. Lane 5, minus RT control; lane 6, blank. The Journal of Molecular Diagnostics 2011 13, 213-219DOI: (10.1016/j.jmoldx.2010.10.009) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions