Functional RNAs expand the synthetic biological toolbox

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Presentation transcript:

Functional RNAs expand the synthetic biological toolbox Isaacs et al., Nat. Biotechnol. 2006

Engineered translation regulators Isaacs et al., Nat. Biotechnol. 2004

Translation riboregulator pairs are specific making the technique scalable Isaacs et al., Nat. Biotechnol. 2004

Allosteric riboregulators of gene expression Base-pairing specificity = scalability Bayer and Smolke., Nat. Biotechnol. 2005

Antiswitches are modular and tunable Bayer and Smolke., Nat. Biotechnol. 2005

Riboswitches – Natural Translational Control Tabor et al., Biotech and Gen Eng. Rev, 2006

Riboswitches can regulate via Boolean logic Sudarsan et al., Science 2006

Regulation of protein decay – postranslational control -ssrA proteolysis system Tag sequence: NH3-Protein-AANDENYALAA-COOH Andersen et al., Appl. Environ. Micro., 1998

Standard Assembly (Combining biobricks)

Build a synthetic gene expression system with XOR logic Mayo et al., PLoS Biology, 2006