Detection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools S. Bonot, L. Ogorzaly,

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Presentation transcript:

Detection of small amounts of human adenoviruses in stools: comparison of a new immuno real-time PCR assay with classical tools S. Bonot, L. Ogorzaly, B. El Moualij, W. Zorzi, H.-M. Cauchie Clinical Microbiology and Infection Volume 20, Issue 12, Pages O1010-O1016 (December 2014) DOI: 10.1111/1469-0691.12768 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Schematical representation of the iPCR procedure and duration of each step. The general setup of iPCR is similar to that of antigen detection through ELISA. The DNA reporter is amplified by real-time PCR for signal generation. The duration of the whole iPCR workflow is about 6 h, including the required time for all the washing steps. Clinical Microbiology and Infection 2014 20, O1010-O1016DOI: (10.1111/1469-0691.12768) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Standard curves obtained by the optimized iPCR for HAdV-2 and HAdV-41 strains. The mean Ct for each dilution was determined from 10 replicates. Clinical Microbiology and Infection 2014 20, O1010-O1016DOI: (10.1111/1469-0691.12768) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Efficiency of HAdV-2 and HAdV-41 detection in spiked stools by ELISA, iPCR and qPCR (n = 9). The values in the histograms correspond to the recovery rate (in %) of each assay for all the virus concentrations tested. *detectable but not quantifiable, **under the detection limit. Clinical Microbiology and Infection 2014 20, O1010-O1016DOI: (10.1111/1469-0691.12768) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions