Column and mobile phase types for basic drugs

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Presentation transcript:

Column and mobile phase types for basic drugs Derivatization of silica to obtain reversed phase columns

Column and mobile phase types for basic drugs The derivatization is not complete because of the complex structure of the silica and the steric hindrance of the alkyl groups. Leading to ------------------- underivatised silanols

Column and mobile phase types for basic drugs Free silanol group gives uncontrollable interaction with the basic compounds

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs Mobile phase can affect the characteristics of the column

Column and mobile phase types for basic drugs Mobile phase can affect the characteristics of the column

Column and mobile phase types for basic drugs Ion pairing agent at low concentrations

Column and mobile phase types for basic drugs Ion pairing agent at high concentrations

Column and mobile phase types for basic drugs Embedded group technology

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs

Column and mobile phase types for basic drugs Poly derivatization

Column and mobile phase types for basic drugs