Volume 37, Issue 3, Pages 451-462 (September 2012) The BH3-Only Proteins Bim and Puma Cooperate to Impose Deletional Tolerance of Organ-Specific Antigens  Daniel H.D. Gray, Fiona Kupresanin, Stuart P. Berzins, Marco J. Herold, Lorraine A. O'Reilly, Philippe Bouillet, Andreas Strasser  Immunity  Volume 37, Issue 3, Pages 451-462 (September 2012) DOI: 10.1016/j.immuni.2012.05.030 Copyright © 2012 Elsevier Inc. Terms and Conditions

Figure 1 Compound Loss of Bim and Other BH3-Only Proteins Can Cause Organ-Specific Autoimmune Disease (A) H&E-stained sections of tissues from lethally irradiated Rag1−/− mice reconstituted with hematopoietic precursors from WT or Vav-BCL-2-transgenic mice, or mice lacking the indicated BH3-only proteins. Arrows indicate areas of lymphocytic infiltration. (B) Immunofluorescent staining of Rag1−/− mouse tissues with serum from Rag1−/− mice reconstituted with hematopoietic precursors from WT or Vav-BCL-2-transgenic mice, or mice lacking the indicated BH3-only proteins. Autoantibody reactivity is shown in green and nuclear DAPI staining in blue. Images are representative of n = 4 mice per group. KO, knockout; DKO, double knockout; ND, no data. Immunity 2012 37, 451-462DOI: (10.1016/j.immuni.2012.05.030) Copyright © 2012 Elsevier Inc. Terms and Conditions

Figure 2 Spontaneous Organ-Specific Autoimmunity in Bbc3−/−Bcl2l11−/− Mice (A) Graph of the cumulative clinical scores of ten organs quantifying autoimmune infiltration and destruction in aged mice deficient in the proteins indicated. Groups were compared using an ANOVA with a multiple-comparison Tukey's post hoc test. ∗p < 0.05, ∗∗∗p < 0.001. Mean ages at time of analysis were: Aire−/−, 237 days (d237) (n = 11); Pmaip1−/−, d147 (n = 9); Bad−/−, d165 (n = 7); Bcl2l11−/−, d141 (n = 8); Bad−/−Bcl2l11−/−, d166 (n = 5); Pmaip1−/−Bcl2l11−/−, d153 (n = 13); and Bbc3−/−Bcl2l11−/−, d143 (n = 15). (B) Representative images of H&E-stained sections of tissues taken from aged Bbc3−/−Bcl2l11−/− mice, with arrows indicating lymphocytic infiltration. (C) Images of H&E-stained sections of pancreas taken from Rag1−/− mice that had been injected with T cells from WT or healthy Bbc3−/−Bcl2l11−/− mice. (D) Graph of the incidence and severity of pancreatitis in Rag1−/− mice that had been injected with T cells from WT or Bbc3−/−Bcl2l11−/− mice (n = 8). (E) CD44 versus CD62L expression on CD4+ splenic T cells, with the mean proportion of activated CD44hiCD62Llo/− T cells shown. Immunity 2012 37, 451-462DOI: (10.1016/j.immuni.2012.05.030) Copyright © 2012 Elsevier Inc. Terms and Conditions

Figure 3 Only the Additional Loss of Puma Exacerbates Defects in Thymocyte Development in Bim-Deficient Mice (A) CD4 versus CD8 expression profiles of thymocytes from lethally irradiated mice reconstituted with hematopoietic progenitors lacking the BH3-only proteins indicated and analyzed 8 weeks later. Profiles are representative of four experiments (total n = 6–13). TKO, triple knockout. (B) Thymocyte subset proportions in mice reconstituted with hematopoietic progenitors from mice lacking the BH3-only proteins indicated (means ± SEM). Statistically significant differences among the proportions of DP thymocytes are shown, tested with a one-way ANOVA with multiple-comparison Tukey's post hoc test (∗∗∗p < 0.0001). (C) CD5 versus CD69 expression on DP thymocytes with the mean (± SEM) proportion of CD69+CD5hi thymocytes shown. (D) CD69 versus CD24 expression on CD4SP thymocytes with the mean (± SEM) proportion of mature CD69−CD24lo/− thymocytes shown. (E) Qa2 versus CD24 expression on CD4SP thymocytes with the mean (± SEM) proportion of mature Qa2+CD24lo/− thymocytes shown. (F) Graph of the numbers of FITC+ recent thymic emigrant (RTE) cells recovered from the spleens of mice 24 hr after intrathymic FITC injection, with means ± SEM shown. Immunity 2012 37, 451-462DOI: (10.1016/j.immuni.2012.05.030) Copyright © 2012 Elsevier Inc. Terms and Conditions

Figure 4 Bim and Puma Are Required for Deletion of OT-I Thymocytes in RIP-mOVA+ Mice (A) Representative dot plots of CD4 and CD8 expression gated on H2-Kb-OVA tetramer+ thymocytes from WT or RIP-mOVA+ chimeras that had been reconstituted with BM from OT-I transgenic mice on WT, Bim-deficient, or Puma- and Bim-double-deficient backgrounds. The percentages of DP, CD4SP, and CD8SP gated on H2-Kb-OVA+ thymocytes are shown. (B and C) Mean percentages (B) and cell numbers (C) of H2-Kb-OVA+ CD8SP thymocytes (± SEM) from the various chimeras. (D) H2-Kb-OVA versus CD8 staining on splenocytes from the various chimeras, with the proportion of H2-Kb-OVA+ CD8+ T cells shown. (E and F) Mean percentages (E) and numbers (F) of H2-Kb-OVA+ CD8+ splenocytes (± SEM) from the various chimeras. PB denotes Puma and Bim double deficient. Groups composed of three to four mice were compared using Student's t test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Data from one of two experiments are shown. Immunity 2012 37, 451-462DOI: (10.1016/j.immuni.2012.05.030) Copyright © 2012 Elsevier Inc. Terms and Conditions

Figure 5 Both Bim and Puma Are Required for the Deletion of Mature CD4SP OT-II Thymocytes in RIP-mOVA+ Mice (A) Representative dot plots of CD4 and CD8 expression gated on Vα2/Vβ5+ thymocytes from RIP-mOVA− or RIP-mOVA+ chimeras that had been reconstituted with BM from OT-II transgenic mice on WT, Bim-deficient, or Puma- and Bim-double-deficient backgrounds. The percentages of Vα2/Vβ5+ CD4SP thymocytes are shown. (B and C) Mean percentages and cell numbers of Vα2/Vβ5+ CD4SP thymocytes (B) or Vα2/Vβ5+CD4+ splenocytes (C) (± SEM) from the various chimeras. (D) CD24 versus Qa2 expression on Vα2/Vβ5+ CD4SP thymocytes from the various chimeras. Groups composed of three to four mice were compared using Student's t test; ∗p < 0.05, ∗∗p < 0.01. Data from one of two experiments are shown. Immunity 2012 37, 451-462DOI: (10.1016/j.immuni.2012.05.030) Copyright © 2012 Elsevier Inc. Terms and Conditions