Nehad Abdulgader Shaer Pathophysiology

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Can crude alkaloids extract of Rhazya stricta induce apoptosis in pancreatic cancer: In vitro study? Nehad Abdulgader Shaer Pathophysiology Volume 26, Issue 1, Pages 97-101 (March 2019) DOI: 10.1016/j.pathophys.2018.09.001 Copyright © 2018 Terms and Conditions

Fig. 1 Showed the cytotoxic effect of different concentrations of R. Strictacrude alkaloidon the normal Wish cells and AsPC-1 and PANC-1 pancreatic cancer cells after 24 h respectively, by MTT assay. Pathophysiology 2019 26, 97-101DOI: (10.1016/j.pathophys.2018.09.001) Copyright © 2018 Terms and Conditions

Fig. 2 Analysis of R. Stricta crude alkaloid extract induced apoptosis in PANC-1 cells by flow cytometry using the TUNEL assay. a) Cells were treated with solvent control, b) Cells were treated with 100 μg/ml and c) Cells were treated with 10 μg/ml. Subsequently, apoptotic cells were labeled and analyzed as described in material and methods section. Pathophysiology 2019 26, 97-101DOI: (10.1016/j.pathophys.2018.09.001) Copyright © 2018 Terms and Conditions

Fig. 3 Analysis of RS induced apoptosis in AsPC-1 cells by flow cytometry using the TUNEL assay. a) Cells were treated with solvent control, b) Cells were treated with 10 μg/ml and c) Cells were treated with 100 μg/ml for 24 h. Subsequently, apoptotic cells were labeled and analyzed as described in material and methods section. Pathophysiology 2019 26, 97-101DOI: (10.1016/j.pathophys.2018.09.001) Copyright © 2018 Terms and Conditions

Fig. 4 Show the mRNA expression by qPCR. (A) And (B) p53 mRNA expression in PANC-1 and AsPC-1 cells under indicated doses of the alkaloid crude extract. (C) And (D) Bcl-2 mRNA expression in PANC-1 and AsPC-1 cellsunder the same experimental conditions. Pathophysiology 2019 26, 97-101DOI: (10.1016/j.pathophys.2018.09.001) Copyright © 2018 Terms and Conditions