Investigating the effects of different library preparation protocols on STR sequencing  S. Riman, H. Iyer, L. Borsuk, K. Gettings, P. Vallone  Forensic Science International: Genetics Supplement Series  Volume 6, Pages e418-e420 (December 2017) DOI: 10.1016/j.fsigss.2017.09.155 Copyright © 2017 Terms and Conditions

Fig. 1 Bead-based PCR cleanup had lower dropout events than column-based PCR cleanup with 30pg of input DNA. Heatmaps summarizing the effect of two different PCR cleanup technologies on total dropout events. STR regions were amplified from S1 and S2 at 30pg DNA input, in duplicate. PCR products were then purified with either column-based (left panel) or bead-based (right panel) cleanup technologies and then subjected to library construction using either the KAPA Hyper (upper left and right panel) or TruSeq DNA PCR-free workflow (lower left and right panel). Analysis of dropout events and heatmaps were generated using the dropout module of the R-package strvalidator [8,9]. Green represents no dropout events. Lavender squares highlight allele dropout where one of the expected alleles is missing. Purple indicates locus drop-out where both expected alleles are absent. Each column represents loci information from a single sample. Forensic Science International: Genetics Supplement Series 2017 6, e418-e420DOI: (10.1016/j.fsigss.2017.09.155) Copyright © 2017 Terms and Conditions