Next-Generation Sequencing for Infectious Disease Diagnosis and Management Martina I. Lefterova, Carlos J. Suarez, Niaz Banaei, Benjamin A. Pinsky The.

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Next-Generation Sequencing for Infectious Disease Diagnosis and Management Martina I. Lefterova, Carlos J. Suarez, Niaz Banaei, Benjamin A. Pinsky The Journal of Molecular Diagnostics Volume 17, Issue 6, Pages 623-634 (November 2015) DOI: 10.1016/j.jmoldx.2015.07.004 Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Illustration of sequencing approaches for diagnosis and monitoring of infectious diseases. Targeted amplicon sequencing uses target-specific primers for template (green bars) enrichment, followed by primers that are partially complementary to the target-specific primers (black bars) and contain sequencing adaptors and bar codes (blue bars). Whole-genome sequencing uses enzymatic or mechanical fragmentation, followed by end repair to allow ligation of primers that contain sequencing adaptors and bar codes (blue bars). Size selection allows only fragments of a predefined length to be used for sequencing. Bioinformatics removal of human sequences is required because the nucleic acids of the organism of interest frequently constitute <1% of the nucleic acid pool. Fragmentation libraries may also be made from PCR-enriched amplicons. The Journal of Molecular Diagnostics 2015 17, 623-634DOI: (10.1016/j.jmoldx.2015.07.004) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions