Volume 33, Issue 1, Pages (April 2015)

Slides:



Advertisements
Similar presentations
Volume 34, Issue 6, Pages (September 2015)
Advertisements

Volume 11, Issue 3, Pages (September 2006)
Volume 23, Issue 6, Pages (December 2012)
Volume 35, Issue 2, Pages (October 2015)
Volume 15, Issue 8, Pages (May 2016)
Volume 36, Issue 5, Pages (March 2016)
Roger B. Deal, Steven Henikoff  Developmental Cell 
Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine  Jin Xu, Tienan Wang, Yi Wu, Wan Jin,
Wnt/β-Catenin and Fgf Signaling Control Collective Cell Migration by Restricting Chemokine Receptor Expression  Andy Aman, Tatjana Piotrowski  Developmental.
Volume 29, Issue 1, Pages (April 2014)
Volume 19, Issue 2, Pages (August 2010)
Volume 21, Issue 4, Pages (October 2011)
Volume 26, Issue 4, Pages (August 2013)
Ectodermal Smad4 and p38 MAPK Are Functionally Redundant in Mediating TGF- β/BMP Signaling during Tooth and Palate Development  Xun Xu, Jun Han, Yoshihiro.
Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish  Chuan Yan, Xiaojing Huo, Shu Wang,
Volume 34, Issue 6, Pages (September 2015)
Redox Regulation by Pitx2 and Pitx3 Is Critical for Fetal Myogenesis
Volume 57, Issue 2, Pages (January 2015)
Volume 26, Issue 5, Pages (September 2013)
Shaping BMP Morphogen Gradients through Enzyme-Substrate Interactions
Volume 26, Issue 5, Pages (September 2013)
Matthew P. Harris, Sean M. Hasso, Mark W.J. Ferguson, John F. Fallon 
Kim F. Rewitz, Naoki Yamanaka, Michael B. O'Connor  Developmental Cell 
Kaoru Sugimoto, Yuling Jiao, Elliot M. Meyerowitz  Developmental Cell 
SoxE Factors Function Equivalently during Neural Crest and Inner Ear Development and Their Activity Is Regulated by SUMOylation  Kimberly M. Taylor, Carole.
The Intracellular Domain of the Frazzled/DCC Receptor Is a Transcription Factor Required for Commissural Axon Guidance  Alexandra Neuhaus-Follini, Greg J.
Jianjun Sun, Wu-Min Deng  Developmental Cell 
Volume 36, Issue 2, Pages (January 2016)
Volume 44, Issue 2, Pages e5 (January 2018)
Volume 14, Issue 4, Pages (April 2008)
Volume 41, Issue 4, Pages e5 (May 2017)
Volume 23, Issue 5, Pages (November 2012)
Jungmook Lyu, Vicky Yamamoto, Wange Lu  Developmental Cell 
Volume 27, Issue 5, Pages (December 2013)
Volume 12, Issue 4, Pages (April 2007)
Volume 9, Issue 2, Pages (October 2014)
Volume 22, Issue 2, Pages (February 2012)
The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin  Jennifer Landino, Ryoma.
Derivation and FACS-Mediated Purification of PAX3+/PAX7+ Skeletal Muscle Precursors from Human Pluripotent Stem Cells  Bianca Borchin, Joseph Chen, Tiziano.
Volume 15, Issue 4, Pages (October 2008)
Marcos Simões-Costa, Michael Stone, Marianne E. Bronner 
Volume 23, Issue 2, Pages (August 2012)
Volume 10, Issue 4, Pages (April 2006)
The BMP Signaling Gradient Patterns Dorsoventral Tissues in a Temporally Progressive Manner along the Anteroposterior Axis  Jennifer A. Tucker, Keith.
Volume 7, Issue 1, Pages (January 2008)
Volume 13, Issue 4, Pages (April 2013)
Volume 25, Issue 6, Pages (June 2013)
Volume 42, Issue 2, Pages e3 (July 2017)
Volume 27, Issue 5, Pages (December 2013)
Volume 12, Issue 12, Pages (September 2015)
Identification of White Adipocyte Progenitor Cells In Vivo
Let-7-Complex MicroRNAs Regulate the Temporal Identity of Drosophila Mushroom Body Neurons via chinmo  Yen-Chi Wu, Ching-Huan Chen, Adam Mercer, Nicholas S.
Volume 20, Issue 4, Pages (April 2011)
Drosophila ASPP Regulates C-Terminal Src Kinase Activity
Telomeric Noncoding RNA TERRA Is Induced by Telomere Shortening to Nucleate Telomerase Molecules at Short Telomeres  Emilio Cusanelli, Carmina Angelica Perez.
The Hippo Pathway Regulates the bantam microRNA to Control Cell Proliferation and Apoptosis in Drosophila  Barry J. Thompson, Stephen M. Cohen  Cell 
Drosophila Maelstrom Ensures Proper Germline Stem Cell Lineage Differentiation by Repressing microRNA-7  Jun Wei Pek, Ai Khim Lim, Toshie Kai  Developmental.
Volume 10, Issue 4, Pages (April 2006)
Volume 8, Issue 4, Pages (April 2005)
Short Telomeres in ESCs Lead to Unstable Differentiation
Volume 4, Issue 4, Pages (April 2009)
Volume 10, Issue 5, Pages (May 2006)
Islet Coordinately Regulates Motor Axon Guidance and Dendrite Targeting through the Frazzled/DCC Receptor  Celine Santiago, Greg J. Bashaw  Cell Reports 
FGF9 Suppresses Meiosis and Promotes Male Germ Cell Fate in Mice
Masakazu Hashimoto, Hiroshi Sasaki
Volume 34, Issue 4, Pages (August 2015)
Cellular Heterogeneity in the Mouse Esophagus Implicates the Presence of a Nonquiescent Epithelial Stem Cell Population  Aaron D. DeWard, Julie Cramer,
Volume 12, Issue 4, Pages (April 2007)
Rab3 Dynamically Controls Protein Composition at Active Zones
Zhen Zhang, Jamie M. Verheyden, John A. Hassell, Xin Sun 
Presentation transcript:

Volume 33, Issue 1, Pages 56-66 (April 2015) Pax3 and Pax7 Play Essential Safeguard Functions against Environmental Stress- Induced Birth Defects  Antoine Zalc, Revital Rattenbach, Frédéric Auradé, Bruno Cadot, Frédéric Relaix  Developmental Cell  Volume 33, Issue 1, Pages 56-66 (April 2015) DOI: 10.1016/j.devcel.2015.02.006 Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 1 Pax3 and Pax7 Are Essential for Facial Development (A) Histological transverse sections across the head regions of E11.5 Pax3GFP/+; Pax7LacZ/+, Pax3GFP/GFP; Pax7LacZ/LacZ, and Pax3Pax3-ERD/GFP embryos at two distinct levels of the nasal process, as indicated by black lines in the embryo scheme. Hematoxylin eosin (H/E); lateral nasal process (LNP); medial nasal process (MNP); nasal pit (NP); neuroepithelium (NE); olfactory epithelium (OE); and eye (E). Scale bar, 500 μm. (B) Bright field and GFP expression (top and middle panels, facial views) of E13.5 embryos of Pax3GFP/+; Pax7LacZ/+, Pax3GFP/GFP; Pax7LacZ/LacZ, and Pax3Pax3-ERD/GFP embryos. Arrowheads represent normally formed nasal processes. White stars indicate rudimentary nasal processes. Bottom panels show histological transverse sections through the nasal processes of these embryos, as indicated by the black line in the embryo scheme. Black stars indicate divided nasal septum. Scale bars, 500 μm. (C) GFP expression in E9.5 Pax3GFP/+; Pax7LacZ/+, Pax3GFP/GFP; Pax7LacZ/LacZ, and Pax3Pax3-ERD/GFP embryos (lateral views). Arrowheads indicate the CNCC migrating into the facial prominences. Scale bar, 200 μm. Developmental Cell 2015 33, 56-66DOI: (10.1016/j.devcel.2015.02.006) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 2 Pax3Pax3-ERD/GFP Embryos Present a Fully Penetrant Frontal Cleft Face Phenotype (A) Immunostaining showing the expression of AP2α in red, PAX7 in white, and GFP in green on transverse sections within the cranial regions of Pax3GFP/+ and Pax3Pax3-ERD/GFP embryos at E9.5. Scale bar, 50 μm. (B and C) Quantification of the proportion of AP2α+ (B) and SOX9+ (C) cells within the PAX3+ population found on transverse sections through the cranial regions of E9.5 PaxGFP/+ and Pax3Pax3-ERD/GFP embryos. Error bars represent the SD. (D) Percentage of Cleaved-CASPASE-3+ cells in the PAX3+ CNCC population of E11.5 embryos of the indicated genotype. Error bars represent the SD. (E) Whole mount in situ hybridization for Msx1 and Dlx2 transcripts on E11.5 embryos of the indicated genotype. Arrowhead indicates Dlx2 expression. Star shows its absence. Dotted lines indicate nasal pit location. Lateral nasal process (LNP), medial nasal process (MNP), maxillary process (Mx), and mandibular process (Md). Scale bars, 500 μm. (F) Composite images of multiple fields showing the oral cavity of DAPI stained E15.5 embryos of the indicated genotype from which the lower jaw was removed. White arrowheads indicate the remaining of the primary palate. Black arrowheads indicate the bifurcated secondary palate. Maxillary process (Mx), prominent rugae (pr), primary palate (pp), secondary palate (sp), site of apposition and fusion of palatal shelves (saf), and primordia of vibrissae (v). Scale bar, 500 μm. Developmental Cell 2015 33, 56-66DOI: (10.1016/j.devcel.2015.02.006) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 3 Impaired Pax3/7 Function Leads to AHR Signaling Upregulation (A) Volcano plot showing the 44 downregulated (DR) genes (green) and 76 upregulated (UR) genes (red) in GFP+ cells FACS-isolated from dissected facial prominences of E11.5 Pax3Pax3-ERD/GFP compared to Pax3GFP/+ control embryos. Blue dots indicate the position of Ahr, Aldh1a3, and Cdkn1a (p21) transcripts. (B) Relative expression of Ahr, Aldhla3, and p21 assayed by RT-qPCR in cells FACS-sorted for GFP from E11.5 embryos of the indicated genotype. Error bars represent the SD. (C) Composite images of multiple fields showing AHR expression in red and GFP in green on transverse sections within the cranial regions of E11.5 embryos of the indicated genotype. Scale bars, 50 μm. Olfactory epithelium (OE), lateral nasal process (LNP) and medial nasal process (MNP). (D) Whole mount in situ hybridization for Aldh1a3 and Fgf8 transcripts on Pax3Pax3-ERD/GFP compared to Pax3GFP/+ control embryos. Stars indicate increased area of transcripts expression for Aldh1a3 or reduced expression for Fgf8. Scale bar, 500 μm. (E) Aldh1a3 and p21 relative expression assayed by RT-qPCR in CNCC from E10.5 WT embryos, exposed to TCDD or carrier as indicated. Error bars represent the SD. Developmental Cell 2015 33, 56-66DOI: (10.1016/j.devcel.2015.02.006) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 4 Impairing Pax3/7 Function Induces Cell-Cycle Exit of CNCC (A and B) Quantification of the number of PAX7+ cells within the GFP+ population on transverse sections of the cranial structures of E9.5 and E10.5 embryos of the indicated genotype (B) GFP expression in E11.5 embryos of the indicated genotypes (lateral views). Scale bar, 500 μm. Red boxes delineate the facial prominences dissected to perform the FACS-sorting in (C). Error bars represent the SD. (C) Percentage of FACS-sorted GFP+ cells within the total population of cells from the dissected facial prominences of E11.5 embryos of the indicated genotype. Error bars represent the SD. (D and E) Phospho-HISTONE-3 (PH3) and p21 expression in red, PAX7 in white, and GFP in green on transverse sections within the cranial regions of embryos at indicated genotypes and stages. Scale bars, 50 μm. (F–I) Quantification of the proportion of PH3+ (F), p21+ (G), AP2α+ (H), and SOX9+ (I) cells within the PAX7+ population in sections through the cranial regions of embryos with the stages and genotypes indicated. Error bars represent the SD. Developmental Cell 2015 33, 56-66DOI: (10.1016/j.devcel.2015.02.006) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 5 Genetic Interaction between Pax3 and AHR Signaling Is Essential for Normal Craniofacial Development (A) Bright field and GFP expression of E13.5 embryos of the indicated genotype treated with DMSO or α-naphthoflavone (facial views, top and middle panels). Arrowheads indicate divided nasal processes. Star marks its fusion. Bottom panels represent composite images of multiple fields showing histological transverse sections through the nasal processes of these embryos. Arrowheads indicate divided nasal septum. Star marks its fusion. Scale bars, 500 μm. (B) Composite images of multiple fields showing the oral cavity of DAPI stained E15.5 embryos of the indicated genotype treated with DMSO or α-naphthoflavone from which the lower jaw was removed. White arrowheads indicate the remaining of the primary palate. Black arrowheads indicate the bifurcated secondary palate. Star marks the alignment of the rugae in the secondary palate of α-naphthoflavone-treated Pax3Pax3-ERD/GFP embryos. Maxillary process (Mx), prominent rugae (pr), primary palate (pp), secondary palate (sp), site of apposition and fusion of palatal shelves (saf), and primordia of vibrissae (v). Scale bar, 500 μm. (C) Number of untreated, DMSO-, and α-naphthoflavone-treated Pax3Pax3-ERD/GFP embryos presenting with a frontal cleft face or a closed face with the stages indicated. At E11.5, for each embryo, classification of the α-naphthoflavone-treated Pax3Pax3-ERD/GFP embryos was based on the nasal process fusion (not occurring in non-rescued mutants) and the level of p21 expression (upregulated in non-rescued mutants compare to control embryos). (D) Percentage of PH3+ cells within the PAX7+ CNCC population in E11.5 embryos of the indicated genotype treated with DMSO or α-naphthoflavone. Error bars represent the SD. (E) p21 relative expression assayed by RT-qPCR in dissected faces of independent E11.5 embryos of the indicated genotype treated with DMSO or α-naphthoflavone. Dotted line represents p21 expression level in DMSO control embryos. Developmental Cell 2015 33, 56-66DOI: (10.1016/j.devcel.2015.02.006) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 6 PAX3 Plays a Safeguard Function against TCDD-Induced Craniofacial Defects (A) p21 relative expression assayed by RT-qPCR in dissected faces of independent E11.5 embryos of the indicated genotype treated with carrier or TCDD. Dotted line represents p21 expression level in carrier-treated control embryos. (B) Bright field and GFP expression of E13.5 embryos of the indicated genotype treated with carrier or TCDD (facial views, top and middle panels). Arrowheads indicate divided nasal processes. Scale bar, 500 μm. (C) Number of carrier- and TCDD-treated E13.5 embryos presenting with a normal or a frontal cleft with the genotype indicated. Developmental Cell 2015 33, 56-66DOI: (10.1016/j.devcel.2015.02.006) Copyright © 2015 Elsevier Inc. Terms and Conditions