Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma Esra Nutku, MDa, Abdelilah Soussi Gounni,

Slides:

Advertisements

Similar presentations

Are leukocytes in asthmatic patients aging faster

Advertisements

Bronchial subepithelial fibrosis and expression of matrix metalloproteinase-9 in asthmatic airway inflammation Makoto Hoshino, MD, Yutaka Nakamura, MD,

Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma Makoto Hoshino, MDa, Yutaka Nakamura, MDa,

Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma: The role of nitric oxide Chun-Hua Wang, MDa, Wen-Yih.

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

Eosinophil cationic protein and histamine after intestinal challenge in patients with cow's milk intolerance Ulf Bengtsson, MD, PhDa, Tina W. Knutson,

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Airway epithelial cells in asthma

Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids Kittipong Maneechotesuwan, MD, PhD, Sirinya.

Calcitonin gene-related peptide– and vascular endothelial growth factor–positive inflammatory cells in late-phase allergic skin reactions in atopic subjects

Evidence for a role for IL-5 and eotaxin in activating and recruiting eosinophils in drug- induced cutaneous eruptions Nikhil Yawalkar, MD, Maithili Shrikhande,

Histologic eosinophilic gastritis is a systemic disorder associated with blood and extragastric eosinophilia, TH2 immunity, and a unique gastric transcriptome

Hong Wei Chu, MDa, Monica Kraft, MDa, James E. Krause, PhDb, Michael D

Role of IL-9 in the pathophysiology of allergic diseases

Asthma and increased bronchial responsiveness in elite athletes: Atopy and sport event as risk factors Ilkka J. Helenius, MDa, Heikki O. Tikkanen, MDb,

Deficient prostaglandin E2 production by bronchial fibroblasts of asthmatic patients, with special reference to aspirin-induced asthma Malgorzata Pierzchalska,

Genetic and histologic evidence for autophagy in asthma pathogenesis

Badrul A. Chowdhury, MD, PhD

Hydroxychloroquine improves airflow and lowers circulating IgE levels in subjects with moderate symptomatic asthma B.Lauren Charous, MD, Elkan F. Halpern,

Chronic sinusitis in severe asthma is related to sputum eosinophilia

Pathophysiology of severe asthma

Inflammation of small airways in asthma

Airway smooth muscle remodeling is a dynamic process in severe long-standing asthma Muhannad Hassan, MD, Taisuke Jo, MD, PhD, Paul-André Risse, PhD,

Oral corticosteroids decrease eosinophil and CC chemokine expression but increase neutrophil, IL-8, and IFN-γ–inducible protein 10 expression in asthmatic.

Susan C. Foley, MD, David Préfontaine, MSc, Qutayba Hamid, MD, PhD

Erythema multiforme to phenobarbital: Involvement of eosinophils and T cells expressing the skin homing receptor F.Jesus Gonzalez, PhDa, Maria J. Carvajal,

Chronologic analysis of in situ cytokine expression in mite allergen-induced dermatitis in atopic subjects Nobuo Yamada, MDa, Motoshi Wakugawa, MDa,

IL-11 expression is increased in severe asthma: Association with epithelial cells and eosinophils Eleanor Minshall, PhDa, Jamila Chakir, PhDb, Michel.

Qutayba Hamid, MD, PhD Journal of Allergy and Clinical Immunology

Airway smooth muscle remodeling is a dynamic process in severe long-standing asthma Muhannad Hassan, MD, Taisuke Jo, MD, PhD, Paul-André Risse, PhD,

Expression of IFN-γ–inducible protein; monocyte chemotactic proteins 1, 3, and 4; and eotaxin in TH1- and TH2-mediated lung diseases Deborah Miotto,

Correlation between alcohol-induced asthma and acetaldehyde dehydrogenase-2 genotype Atsuko Takao, MDa, Terufumi Shimoda, MDa, Shigeru Kohno, MDa, Sadahiro.

Circadian variation of sputum inflammatory cells in mild asthma

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

Recruitment of T cells to the lung in response to antigen challenge

Are leukocytes in asthmatic patients aging faster

Effect of ozone and nitrogen dioxide on the release of proinflammatory mediators from bronchial epithelial cells of nonatopic nonasthmatic subjects and.

Molecular virology and immunology of HIV infection

Gross pathology and histopathology of asthma

Vascular endothelial growth factor in patients with acute asthma

IL-9 expression by human eosinophils: Regulation by IL-1β and TNF-α

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

T-cell cytokines in chronic allergic eye disease

TH1/TH2 cytokines and inflammatory cells in skin biopsy specimens from patients with chronic idiopathic urticaria: Comparison with the allergen-induced.

Sarbjit S. Saini, MDa, Jennifer J

A randomized, double-blind trial of the effect of treatment with montelukast on bronchial hyperresponsiveness and serum eosinophilic cationic protein.

Intracellular IL-5 and T-lymphocyte subsets in atopic and nonatopic bronchial asthma Yutaro Shiota, MD, PhDa, Hitomi Arikita, MTa, Naokatsu Horita, MDb,

Molecular pathology of allergic disease

Delayed eosinophil apoptosis in asthma

Seasonal intestinal inflammation in patients with birch pollen allergy

Prognosis and prediction of response to surgery in allergic patients with chronic sinusitis François Lavigne, MDa, Cong Thu Nguyen, MDa, Lisa Cameron,

A case of acute eosinophilic pneumonia with hypersensitivity to “red spider” allergens Bertrand Godeau, MDa, Laurent Brochard, MDb, Ioanis Theodorou,

Rame A. Taha, MDa, Eleanor M. Minshall, PhDa, Donald Y. M

Monocyte chemotactic proteins in allergen-induced inflammation in the nasal mucosa: Effect of topical corticosteroids Pota Christodoulopoulos, BSca,

Local expression of ϵ germline gene transcripts and RNA for the ϵ heavy chain of IgE in the bronchial mucosa in atopic and nonatopic asthma Sun Ying,

Abnormalities of cell and mediator levels in bronchoalveolar lavage fluid of patients with mild asthma Sally E. Wenzel, MD Journal of Allergy and Clinical.

Reply Journal of Allergy and Clinical Immunology

Cytokine profile in minor salivary glands from patients with bronchial asthma Anne Tsicopoulos, MDa, b, Anne Janin, MDc, Hikmat Akoum, PhDa, Catherine.

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

IL-9 and c-Kit+ mast cells in allergic rhinitis during seasonal allergen exposure: Effect of immunotherapy Kayhan T. Nouri-Aria, PhD, FRCPath, Charles.

Peripheral blood and airway tissue expression of transforming growth factor β by neutrophils in asthmatic subjects and normal control subjects Hong Wei.

Comparison of ciliary activity and inflammatory mediator release from bronchial epithelial cells of nonatopic nonasthmatic subjects and atopic asthmatic.

Airway remodeling-associated mediators in moderate to severe asthma: Effect of steroids on TGF-β, IL-11, IL-17, and type I and type III collagen expression

Immunopathology of atopic dermatitis

Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL- 8, and tumor necrosis factor-α by peripheral mononuclear cells of workers.

Differences in airway remodeling between subjects with severe and moderate asthma Carmela Pepe, MD, Susan Foley, MD, Joanne Shannon, MD, Catherine Lemiere,

Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids Kittipong Maneechotesuwan, MD, PhD, Sirinya.

Chitinases and chitinase-like proteins in TH2 inflammation and asthma

Presentation transcript:

Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma Esra Nutku, MDa, Abdelilah Soussi Gounni, PhDa, Ron Olivenstein, MDb, Qutayba Hamid, MD, PhDa Journal of Allergy and Clinical Immunology Volume 106, Issue 2, Pages 288-292 (August 2000) DOI: 10.1067/mai.2000.107932 Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 1 A, A representative example of IL-12 (p40) mRNA in peripheral blood eosinophils from an asthmatic individual. Positive hybridization signals are localized as silver grains (magnification 200×). B, Immunofluorescent staining with an FITC-conjugated antibody against both subunits of IL-12 is shown in peripheral blood eosinophils from asthmatic subjects (magnification 1000×). C, Control experiment (no staining). D, Double-staining experiments to colocalize IL-5 and IL-12 in peripheral blood eosinophils. Arrows indicate the positive double staining. Journal of Allergy and Clinical Immunology 2000 106, 288-292DOI: (10.1067/mai.2000.107932) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 2 A , Mean percentages of p35 and p40 mRNA+ eosinophils in peripheral blood taken from asthmatic subjects (n = 7) and nonasthmatic control subjects (n = 7). There were statistically significantly greater percentages of IL-12 mRNA+ (both p35 and p40) cells in nonasthmatic subjects compared with asthmatic subjects. B , IL-12 immunoreactivity in peripheral blood eosinophils obtained from asthmatic subjects (n = 6), nonasthmatic control subjects (n = 7), and patients with chronic bronchitis (n = 7). The percentage of eosinophils expressing IL-12–immunoreactive protein was lower in asthmatic subjects compared with that found in patients with chronic bronchitis (P < .001) and healthy control subjects (P < .05). Journal of Allergy and Clinical Immunology 2000 106, 288-292DOI: (10.1067/mai.2000.107932) Copyright © 2000 Mosby, Inc. Terms and Conditions

Fig. 3 A representative graph demonstrating the percentages of eosinophils positive for IL-12 and IL-5 in asthmatic and control subjects (P < .001). Journal of Allergy and Clinical Immunology 2000 106, 288-292DOI: (10.1067/mai.2000.107932) Copyright © 2000 Mosby, Inc. Terms and Conditions