The Detection of t(14;18) in Archival Lymph Nodes

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Presentation transcript:

The Detection of t(14;18) in Archival Lymph Nodes Sharon L. Barrans, Paul A.S. Evans, Sheila J.M. O'Connor, Roger G. Owen, Gareth J. Morgan, Andrew S. Jack The Journal of Molecular Diagnostics Volume 5, Issue 3, Pages 168-175 (August 2003) DOI: 10.1016/S1525-1578(10)60469-2 Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Representative FISH images of the pattern of staining of the BCL2/IgH probe set. a: Normal pattern of staining. Each cell nucleus has two red (BCL2) and two green (IgH) FISH signals, one for each copy of the chromosome. The counterstain is DAPI, which is visualized as blue fluorescence. b: A case with a t(14;18). Each nucleus has three signals with both BCL2 and IgH probe sets, indicating a “split” in one each of the copies of the genes. The reciprocal translocation, t(14;18), is demonstrated by the presence of two fusion signals in each cell (indicated by yellow arrows), along with a residual normal copy of each gene. The counterstain is DAPI. The Journal of Molecular Diagnostics 2003 5, 168-175DOI: (10.1016/S1525-1578(10)60469-2) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 t(14;18) multiplex PCR analysis on DNA extracted from 28 frozen biopsies of FL. a: MBR multiplex (JH + MBR): 15 of 28 FL cases had a visible band and were classed as positive (+). b: mcr multiplex (JH + mcr, 5′mcr, 3′MBR): 8 of 28 FL cases had a visible band and were classed as positive (+). The Journal of Molecular Diagnostics 2003 5, 168-175DOI: (10.1016/S1525-1578(10)60469-2) Copyright © 2003 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions