Der p 11 Is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis Srinita Banerjee, Yvonne Resch, Kuan-Wei Chen, Ines.

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Der p 11 Is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis Srinita Banerjee, Yvonne Resch, Kuan-Wei Chen, Ines Swoboda, Margit Focke-Tejkl, Katharina Blatt, Natalija Novak, Magnus Wickman, Marianne van Hage, Rosetta Ferrara, Adriano Mari, Ashok Purohit, Gabrielle Pauli, Elopy N. Sibanda, Portia Ndlovu, Wayne R. Thomas, Vladislav Krzyzanek, Sebastian Tacke, Ursula Malkus, Peter Valent, Rudolf Valenta, Susanne Vrtala Journal of Investigative Dermatology Volume 135, Issue 1, Pages 102-109 (January 2015) DOI: 10.1038/jid.2014.271 Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Purification of natural and recombinant Der p 11. (a) A molecular mass marker (M) and 3 μg of purified rDer p 11 and nDer p 11 were separated by SDS-PAGE under reducing and nonreducing conditions and were silver stained. rDer p 11 bands marked with an arrow were subjected to mass spectrometry analysis. (b) Circular dichroism analysis. The molecular ellipticities (θ) (y axis) at different wavelengths (200–260 nm, x axis) are displayed for rDer p 11 (gray) and nDer p 11 (black). Journal of Investigative Dermatology 2015 135, 102-109DOI: (10.1038/jid.2014.271) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Occurence of Der p 11 in mite bodies. (a) Nitrocellulose-blotted extracts from D. pteronyssinus bodies (left panel) and feces (right panel) were incubated with anti-rDer p 11, anti-rDer p 2 antibodies, or preimmune Ig. Molecular weights (kDa) are indicated. (b) Immunogold electron microscopy. Ultrathin sections from muscle and feces were stained with anti-Der p 11 antiserum (right panel) or preimmune antiserum (left panel). Arrows indicate two of the gold particles localizing Der p 11. Black bars denote the scale. Journal of Investigative Dermatology 2015 135, 102-109DOI: (10.1038/jid.2014.271) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 IgE reactivity of rDer p 11. (a) Comparison of the intensity of IgE recognition of rDer p 11, rDer p 2, and BSA (negative control) as tested by dot blot with sera from mite-allergic patients (1–20), a nonallergic individual (NC), and with buffer alone (BC). (b) Prevalence of IgE reactivity to rDer p 11 and rDer p 2 (y axis: percentages of reactive patients) in different populations of house dust mite-allergic patients (x axis: Austria: n=47; France: n=38; Italy: n=67; Sweden: n=52; Zimbabwe: n=34). Journal of Investigative Dermatology 2015 135, 102-109DOI: (10.1038/jid.2014.271) Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions