Fluorescent amplified fragment length polymorphism genotyping of Salmonella Enteritidis: a method suitable for rapid outbreak recognition  F. Scott, J. Threlfall, J. Stanley, C. Arnold  Clinical Microbiology and Infection  Volume 7, Issue 9, Pages 479-485 (September 2001) DOI: 10.1046/j.1198-743x.2001.00296.x Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 The dendrogram shows the relationship by FAFLP between 25 Salmonella Enteritidis phage type reference strains (PTseries) and 20 PT 6 and 6a isolates (SE series) using the unweighted pair-group method with arithmetic averages (UPGMA). Clinical Microbiology and Infection 2001 7, 479-485DOI: (10.1046/j.1198-743x.2001.00296.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 FAFLP profile comparison of PT 6a and SE 611: (a) primer combination Eco + 0–Mse +TA, fragments from 226 to 280 bp in size; (b) primer combination Eco + 0–Mse +T, fragments from 258 to 296 bp in size. Genotyper v 2.5 electropherograms show that FAFLP has been successfully used to resolve these two strains of PT 6a. Polymorphic amplified fragments included in the study are labeled. (a) There are five amplified fragment differences between PT 6a and SE 611 in the size range 226–280 bp using the selective primer combination Eco + 0–Mse +TA. PT 6a has three amplified fragments not present in SE 611 (sized 232.82 bp, 235.08 bp and 243.16 bp). SE 611 has two amplified fragments not present in PT 4 (sized 249.84 bp and 268.50 bp). (b) There are two amplified fragment differences between PT 6a and SE 611 in the size range 260–296 bp using the selective primer combination Eco + 0–Mse +T. PT 6a has three amplified fragment differences not present in SE 611 (sized 259 bp and 265.74 bp). Clinical Microbiology and Infection 2001 7, 479-485DOI: (10.1046/j.1198-743x.2001.00296.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions