Knockdown of DAO inhibits DNA damage–induced senescence.

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Supplemental Figures A B % Senescent Cells Relative to control *

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Knockdown of DAO inhibits DNA damage–induced senescence. Knockdown of DAO inhibits DNA damage–induced senescence. (A) U2OS cells transfected with siRNAs for DAO (DAO-1 and DAO-2) were treated with 2 μM etoposide for 7 d, and the expression levels of DAO were determined by qPCR. (B) HepG2 cells transfected with siRNAs for DAO (DAO-1 and DAO-2) were treated with 10 μM etoposide for 48 h, and the expression levels of DAO were determined by immunoblot analysis. (C, D) U2OS (C) and HepG2 (D) cells depleted of DAO were treated with 2 and 10 μM etoposide for 7 d and 48 h, respectively, and subjected to SA-β-gal staining. The percentage of SA-β-gal–positive cells (C left panel, D) and representative microscopic images (C right panel) are shown. Bars, 50 μm. (E) U2OS cells depleted of DAO were treated with 2 μM etoposide for 7 d and subjected to colony-formation assay. Relative proliferation rate (upper panel) and representative images (lower panel) are shown. (F) U2OS cells treated as in (E) but for 2 d instead of 7 d were subjected to immunoblot analysis. The protein levels relative to the γ-tubulin levels were quantified using NIH ImageJ software and are indicated at the bottom of each lane. Data are mean ± SD (n = 3 except in (A) where n = 2 independent experiments). Statistical significance is shown using the t test analysis; *P < 0.05, **P < 0.01. Taiki Nagano et al. LSA 2019;2:e201800045 © 2019 Nagano et al.