Analytical Validation of a Personalized Medicine APOL1 Genotyping Assay for Nondiabetic Chronic Kidney Disease Risk Assessment Jinglan Zhang, Anastasia.

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Analytical Validation of a Personalized Medicine APOL1 Genotyping Assay for Nondiabetic Chronic Kidney Disease Risk Assessment Jinglan Zhang, Anastasia Fedick, Stephanie Wasserman, Geping Zhao, Lisa Edelmann, Erwin P. Bottinger, Ruth Kornreich, Stuart A. Scott The Journal of Molecular Diagnostics Volume 18, Issue 2, Pages 260-266 (March 2016) DOI: 10.1016/j.jmoldx.2015.11.003 Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 PCR and multiplex allele-specific primer extension (ASPE) primer locations for APOL1 genotyping and Sanger sequencing. The NG_023228 genomic sequence of the APOL1 gene (http://www.ncbi.nlm.nih.gov/nuccore; accession number NG_023228.1) was used to design all PCR and ASPE primers for APOL1 genotyping and Sanger sequencing. The APOL1 genotyping and Sanger sequencing primer pairs used for amplification were PCR-F1/R1 and PCR-F2/R2, respectively. The six ASPE primers used to detect the WT, G1, or G2 alleles are illustrated with directional arrows and labeled as c.1072WT, c.1072A>G, c.1200WT, c.1200T>G, c.1212_1217WT, and c.1212_1217del6. Primer sequences are listed in Materials and Methods and Table 1. The Journal of Molecular Diagnostics 2016 18, 260-266DOI: (10.1016/j.jmoldx.2015.11.003) Copyright © 2016 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions