Quiz#5 LC710 11/16/11 name___________

Slides:



Advertisements
Similar presentations
A Little More Advanced Biotechnology Tools
Advertisements

Transcription and Translation Flip Book Your Name Your Class Period.
Key Area : Genetic Control of Metabolism in Micro-organisms Unit 2: Metabolism and Survival.
Cloning:Recombinant DNA
PLASMIDS Dr. E.
Bacteria Transformation
Introduction to Biotech Notes MANIPULATING and ANALYZING DNA.
C HAPTER 20 PART 3: A L ITTLE M ORE A DVANCED B IOTECHNOLOGY T OOLS Better Plasmids.
Transformation AP Big Idea #3: Genes and Information Transfer connected with AP Big Idea #1 (Evolution) & #2 (Cellular Processes)
Chapter 13 Genetic Engineering
IV Cell Transformation Recombinant DNA Host Cell DNA Target gene Modified Host Cell DNA.
Plasmids Continued Once we insert the plasmid into the bacteria how do we know its in the bacteria and has the the right gene in it?
Fig Fig Fig Fig Fig
Cloning Genes Gene cloning: amplifying a specific piece of DNA via a bacteria cell Cloning vector: a replicating DNA molecule attached with a foreign DNA.
Gene Regulations and Mutations
Agenda 07/20/2010 Sketch Transcription Quiz – genetics 1 Translation New Vocab Assembly Enzyme Synthesis Review Mutations – Research Genetic Engineering.
Bioengineering Turning Genes on and off in Bacteria.
Genetic Engineering Genetic engineering is also referred to as recombinant DNA technology – new combinations of genetic material are produced by artificially.
DNA in the Cell Stored in Number of Chromosomes (24 in Human Genome) Tightly coiled threads of DNA and Associated Proteins: Chromatin 3 billion bp in Human.
GENETIC ENGINEERING. NUCLEOID DNA the main DNA of an organism found in both prokaryotic and eukaryotic cells Prokaryotes – unprotected Eukaryotes –
DNA Technology & Genomics
 What is different between these 2 sequences? GGAATTCCTAGCAAT CCTTAAGGATCGTTA CTACGTGAGGAATTC GATGCACTCCTTAAG.
Bacterial Plasmids Loops of DNA found in some bacteria
Mutations Learning Goal: Identify mutations in DNA (point mutation and frameshift mutation caused by insertion or deletion) and explain how they can affect.
DNA to Protein. Chromosomes are made of tightly packed DNA A gene is a section of the DNA molecule that codes for a particular protein. The order of nitrogen.
Transformation Lab What are plasmids? Circular sequences of DNA that can be incorporated into a bacterial host genome. What makes them so special?? They.
PGLO Bacterial Genetics. E. Coli structure.
Gene Cloning & Creating DNA Libraries. Клонирование генов Что означает термин «клонирование»? Как происходит клонирование генов? Чем это отличается от.
C HAPTER 20 PART 3: A L ITTLE M ORE A DVANCED B IOTECHNOLOGY T OOLS Better Plasmids.
Markers for genetic engineering ALBIO9700/2006JK.
Genetic Code and Interrupted Gene Chapter 4. Genetic Code and Interrupted Gene Aala A. Abulfaraj.
DNA and Molecular Genetics DNA structure Replication Gene concept Transcription Translation Uses Evidence Life cycle.
Quiz#8 LC710 11/28/11 name___________
A Little More Advanced Biotechnology Tools
Topics to be covers Basic features present on plasmids
Protein Synthesis The formation of proteins based on information in DNA and carried out by RNA. (Gene expression) Flow of Genetic Information: DNA “unzips”
V Cell Transformation Recombinant DNA Host Cell DNA Target gene
Variation among organisms
CHAPTER 20 PART 3: A LITTLE MORE ADVANCED BIOTECHNOLOGY TOOLS
Chapter 13 Section 3 Cell Transformation
“Taiwan Breeds Green Glowing Pigs”
Quiz #4(8%) Biol710 11/05/12 name___________
13-3 Cell Transformation Interactive pgs. 329.
BTY100-Lec#4.2 DNA to Protein (Central Dogma).
National 5 Biology Genetic Engineering Mr G Davidson.
Genetic Control of Metabolism
Gene Expression 1. Gene expression is the activation of a gene that results in transcription and the production of mRNA. Only a fraction of any cell’s.
In: What are INTRONS and EXONS again?
A Little More Advanced Biotechnology Tools
Making Human (eukaryote) proteins in Bacteria (prokaryote)
Quiz#6 LC710 10/13/10 name___________
Quiz#7 LC710 10/18/10 name___________
Copyright Pearson Prentice Hall
Warm Up 10/6: (Accessing old pathways!)
A Little More Advanced Biotechnology Tools
!'!!. = pt >pt > \ ___,..___,..
Section: ___ Time of lab:______8th or 9th floor (circle)
Science Vocabulary Topic: DNA Unit #4 By:
Quiz#1 LC710 10/10/12 name____________
Quiz#6 LC710 10/13/10 name___________
A Little More Advanced Biotechnology Tools
A Little More Advanced Biotechnology Tools
Quiz#1a LC710 11/05/17 name____________
Copyright Pearson Prentice Hall
Metabolism and Survival
Plasmids, Antibiotics & Resistance
Title: Biology 4/10/07 Objectives: Class Topics
Copyright Pearson Prentice Hall
Chapter 10 – The Gene and Protein Synthesis
Cell Transformation.
Presentation transcript:

Quiz#5 LC710 11/16/11 name___________ Q1:Given the following eukaryotic gene A: 1 2 3 4 gene A normally blocks mRNA translation. You want to make a lot of GENE A protein in bacteria. Draw out and annotate your construct (insert only): it is a given that your plasmid has an Origin of replication and a resistance marker. 4pts. Inaccuracies may incur deductions. Let’s say that you don’t want to include exon 1, then how will your construct differ? 2pts. Be Specific! Let’s say that you don’t want to include exon 2, then how will your construct differ? 2pts. Be Specific!