Effects of follicular phase and oocyte–cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella Sheyla.

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Effects of follicular phase and oocyte–cumulus complexes quality on the protein profile and in vitro oocyte meiosis competence in Cebus apella Sheyla F.S. Domingues, Ph.D., Maria C. Caldas-Bussiere, Ph.D., Marilvia D. Petretski, Ph.D., Otávio M. Ohashi, Ph.D., Julianne S. Lima, B.S., Regiane R. Santos, Ph.D., Marcela S. Cordeiro, M.S., Paulo H. Gomes de Castro, M.S. Fertility and Sterility Volume 93, Issue 5, Pages 1662-1667 (March 2010) DOI: 10.1016/j.fertnstert.2009.05.029 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Representative protein profile (SDS-PAGE, 5% to 15%) of Cebus apella oocytes harvested from antral follicles on days 5, 7, and 9. Day 5: lanes 1 and 4, cumulus intact oocytes from dominant follicles; lane 2, cumulus intact oocyte from subordinate follicle; lane 3, denuded oocyte from subordinate follicle. Day 7: lanes 1 and 4, cumulus intact oocytes from dominant follicles; lane 2, denuded oocyte from subordinate follicle; lane 3, cumulus intact oocyte from subordinate follicle. Day 9: lanes 1 and 3, cumulus intact oocytes from subordinate follicles; lane 2, cumulus intact oocyte from dominant follicle; lane 4, cumulus intact oocyte from subordinate follicle. Fertility and Sterility 2010 93, 1662-1667DOI: (10.1016/j.fertnstert.2009.05.029) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Densitometry analysis (SDS-PAGE, 5% to 15%) of two majority proteins in Cebus apella oocytes recovered on days 5 (168 kd) and 7 (192 kd) of the menstrual cycle. Day 5: lane A1, cumulus intact oocyte from dominant follicle; lane A2, cumulus intact oocyte from subordinate follicle; lane A3, denuded oocyte from subordinate follicle; lane A4, cumulus intact oocyte from subordinate follicle. Day 7: lane B1, cumulus intact oocyte from dominant follicle; lane B2, denuded oocyte from subordinate follicle. Fertility and Sterility 2010 93, 1662-1667DOI: (10.1016/j.fertnstert.2009.05.029) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Protein profile (SDS-PAGE, 5% to 15%) of Cebus apella cumulus cells obtained from antral follicles on days 5, 7, and 9 of the menstrual cycle. Day 5: lanes1, 4, and 5, cumulus cells from dominant follicles; lanes 2 and 3, cumulus cells from subordinate follicles. Day 7: lanes 1, 2, and 4, cumulus cells from subordinate follicles; lane 3, cumulus cells from dominant follicle. Day 9: lanes 1, 3, and 5, cumulus cells from dominant follicles; lanes 2 and 4, cumulus cells from subordinate follicles. Fertility and Sterility 2010 93, 1662-1667DOI: (10.1016/j.fertnstert.2009.05.029) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Cebus apella oocyte after 36 hours of in vitro maturation: representative nucleus in metaphase II (black arrow) and first polar body (white arrow) (magnification: ×800). Fertility and Sterility 2010 93, 1662-1667DOI: (10.1016/j.fertnstert.2009.05.029) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 5 Germinal vesicle breakdown (GVBD) and meiotic status of Cebus apella oocytes obtained from subordinate (SF) and dominant (FD) follicles after 24 and 36 hours of in vitro culture. PI, prophase I; AI, anaphase I; TI, telophase I; MII, metaphase II. (a–c) Effect of in vitro maturation (IVM) time on GVBD in oocytes recovered from dominant and subordinate follicles (P<.05). (A–D) Effect of follicular category on nuclear maturation after 24 and 36 hours of IVM (P<.05). ∗Statistically significant difference between subordinate and dominant follicles (P<.05). Fertility and Sterility 2010 93, 1662-1667DOI: (10.1016/j.fertnstert.2009.05.029) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions