Volume 5, Issue 2, Pages 231-241 (February 2000) The Drosophila SHC Adaptor Protein Is Required for Signaling by a Subset of Receptor Tyrosine Kinases  Stefan Luschnig, Jana Krauss, Kerstin Bohmann, Isabelle Desjeux, Christiane Nüsslein-Volhard  Molecular Cell  Volume 5, Issue 2, Pages 231-241 (February 2000) DOI: 10.1016/S1097-2765(00)80419-0

Figure 1 dshc Mutants Show Terminal Defects in the Embryo and a Ventralization of the Eggshell (A and B) Cuticle preparations of a wild-type (A) embryo and dshc111–40 (B) mutant germline clones. The upper embryo in (B) recieved a wild-type copy of dshc from the father; the lower embryo is maternally and zygotically mutant. (C and D) Malpighian tubules seen in a live wild-type (C) embryo are absent in a dshc111–40 (D) embryo. (E and F) tll in situ hybridization in a wild-type (E) and a dshc111–40 (F) embryo. (G and H) A wild-type (G) egg shows two dorsal appendages, while an oocyte from a dshc/dshc (H) female is ventralized and shows a fused appendage. Abbreviations: A7 and A8, abdominal segments 7 and 8; FK, Filzkörper; MT, Malpighian tubules; numbers in (E) and (F) indicate the border of the posterior tll expression domain as percent of egg length, with 0% at the posterior pole. Anterior is to the left in all figures, except where indicated differently. Molecular Cell 2000 5, 231-241DOI: (10.1016/S1097-2765(00)80419-0)

Figure 2 dshc Adults Show Phenotypes Characteristic of Reduced DER Signaling (A and B) SEM micrographs of wild-type (A) and dshcBG/dshcBG (B) eyes are shown at the same magnification. (C and D) Tangential sections through the anterior portion of a wild-type (C) and a dshcBG/dshcBG (D) eye; encircled ommatidia lack photoreceptors. Note the presence of small central photoreceptors in the mutant ommatidia. Arrows indicate ommatidial polarity with the arrowhead resembling the R3 cell; red arrows indicate rotated ommatia. (E and F) Wings of a wild-type (E) and dshcBG/dshcBG (F) animal; note the gap in the L4 vein of the mutant wing. Anterior is up in (E) and (F). Molecular Cell 2000 5, 231-241DOI: (10.1016/S1097-2765(00)80419-0)

Figure 3 Molecular Nature of dshc Mutations (A) The domain structure of DSHC and the position of the YYND motif are indicated below the bar representing the protein. Above, the changes found in EMS-induced alleles are shown; nucleotide and amino acid residue numbers refer to the previously published sequence (Lai et al. 1995; GenBank accession number U26445). (B) DSHC Western blot analysis. A DSHC-specific 45 kDa band is detected in wild type and dshc111–40 but is not detectable in dshcBG mutants. Molecular Cell 2000 5, 231-241DOI: (10.1016/S1097-2765(00)80419-0)

Figure 4 DSHC Acts Downstream of Torso Cuticle preparations from embryos of the following maternal genotypes: Tor4021/+; + (A); Tor4021/+; dshc111–40/+ (B); Tor4021/+; dshc111–40/dshc111–40 (C); Tor4021/+; dosP115/dosP115 (D). Note the appearance of Filzkörper material (FK) and Tuft (Tft) in (B) and the partial suppression of segmentation in (D). Molecular Cell 2000 5, 231-241DOI: (10.1016/S1097-2765(00)80419-0)

Figure 5 DSHC Acts Downstream of DER (A–C) SEM micrographs of eyes of the following genotypes: ElpB1/ElpB1; + (A); ElpB1/ElpB1; dshcBG/+ (B); ElpB1/ElpB1; dshcBG/dshcBG (C). (D–G) Wings and eggs from females of the genotypes UAS–λTOP4.2/+; CY2-Gal4/+; + (D and F); UAS–λTOP4.2/+; CY2-Gal4/+; dshcBG/dshcBG (E and G). Note the suppression of ectopic veins in (E) and the appearance of appendage material on the egg in (G). Anterior is up in (D) and (E). Molecular Cell 2000 5, 231-241DOI: (10.1016/S1097-2765(00)80419-0)

Figure 6 dshc Mutations Do Not Suppress the Effect of Activated Sevenless SEM micrographs (A–C) and tangential sections (D–F) through the posterior portion of eyes of the following genotypes: Sevs11/Y; + (A and D); Sevs11/Y; dshcBG/+ (B and E); Sevs11/Y; dshcBG/dshcBG (C and F). Molecular Cell 2000 5, 231-241DOI: (10.1016/S1097-2765(00)80419-0)

Figure 7 DSHC Acts in Parallel to DOS and DRK Cuticle preparations and tll in situ hybridization pattern of germline clone-derived embryos of the following maternal genotypes: dosP115 FRT2A (A and B); dshcBG FRT2A (C and D); dosP115, dshcBG FRT2A (E and F); drkΔP24 FRTG13; + (G and H); drkΔP24 FRTG13; dshcBG FRT2A (I); drkΔP24 FRTG13; dosP115 FRT2A (J). Numbers in (B), (D), and (H) indicate the border of the posterior tll expression domain as percent of egg length. Molecular Cell 2000 5, 231-241DOI: (10.1016/S1097-2765(00)80419-0)