DNA and RNA References for qRT-PCR Assays in Exfoliated Cervical Cells

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DNA and RNA References for qRT-PCR Assays in Exfoliated Cervical Cells Martin Steinau, Mangalathu S. Rajeevan, Elizabeth R. Unger The Journal of Molecular Diagnostics Volume 8, Issue 1, Pages 113-118 (February 2006) DOI: 10.2353/jmoldx.2006.050088 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Distributions of raw Ct values for each HK gene visualized as boxplots. Boxplots showing the distributions of raw Ct values (arithmetic means of duplicates) of the 30 samples for each of the genes tested. Gray boxes indicate the interquartile range with the median as the black center bar. The first and fourth quartile are shown as the extended vertical bars, and stars and open circles mark outliers and suspected outliers, respectively. The Journal of Molecular Diagnostics 2006 8, 113-118DOI: (10.2353/jmoldx.2006.050088) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Correlation of different references. A: Correlation of the three most stable genes by GeNorm (geometric means of Ct values) and DNA content (Ct of B-globin). B: No correlation was apparent among the three most stable genes (geometric means of Ct values) and total RNA mass estimated by densitometric assessment of the 18S and 28S ribosomal bands. The Journal of Molecular Diagnostics 2006 8, 113-118DOI: (10.2353/jmoldx.2006.050088) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions