Specificity in Trk Receptor:Neurotrophin Interactions

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Specificity in Trk Receptor:Neurotrophin Interactions Mark J Banfield, Ruth L Naylor, Alan G.S Robertson, Shelley J Allen, David Dawbarn, R.Leo Brady Structure Volume 9, Issue 12, Pages 1191-1199 (December 2001) DOI: 10.1016/S0969-2126(01)00681-5

Figure 1 Structure of the TrkB-d5:NT-4/5 Complex Views of the overall structure of the TrkB-d5:NT-4/5 complex are shown, each approximately orthogonal. The asymmetric unit of the crystal contains a single copy of the TrkB-d5:NT-4/5 complex, comprising one homodimer of NT-4/5 bound to two monomers of TrkB-d5. The NT-4/5 homodimer is colored red (one monomer) and blue (the second monomer) with various secondary structure elements labeled. TrkB-d5 monomers are colored cyan, and the β sheets are labeled. Also shown (yellow bonds) is NT-4/5 residue Arg-11 with TrkB residues Asp-298, Cys-302, and Cys-345 (these highlight the location of the specificity interaction site), and also shown (violet bonds) are NT-4/5 residues Gln-94 and Arg-114 with TrkB residues Asp-349 and Asn-350 (which highlight the location of the conserved interaction site). The model for the TrkB-d5:NT-4/5 complex includes residues Gly-1 to Thr-127 for one NT-4/5 monomer and Thr-5 to Gly-128 for the second. TrkB-d5 residues Ala-286 to Gly-383 inclusive are observed in both copies of the receptor domain. Figures 1–4 were all prepared with MOLSCRIPT [43] and RASTER3D [44]. Structure 2001 9, 1191-1199DOI: (10.1016/S0969-2126(01)00681-5)

Figure 2 Comparison of the Known Trk-Neurotrophin Complexes Stereo diagram showing the Cα traces of the TrkB-d5:NT-4/5 (cyan and red, respectively) and TrkA-d5:NGF (biege and blue, respectively) complexes. The structures have been overlaid by superimposing the neurotrophin Cα atoms only. The overall similarity in the architecture of the two complexes is apparent, as is the displacement (calculated as 4° rotation) of the Trk-d5 domains relative to the respective neurotrophin. Structure 2001 9, 1191-1199DOI: (10.1016/S0969-2126(01)00681-5)

Figure 3 Contacts Formed in the TrkB-d5:NT-4/5 Complex Contacts formed at (a) the conserved patch and (b) the specificity patch. Stereo views showing fragments of the TrkB-d5 Cα trace in cyan and those of the NT-4/5 Cα trace in red and blue. Secondary structure features and relevant amino acids are labeled. In (b), TrkB-d5 strands shown are ABED from bottom to top. Bonds in individual residues are shown in khaki for TrkB and light gray for NT-4/5. Intermolecular hydrogen bonds are displayed as dashed lines. Structure 2001 9, 1191-1199DOI: (10.1016/S0969-2126(01)00681-5)

Figure 4 Stereo View Comparing Interactions at the Specificity Patch between the TrkB-d5:NT-4/5 and TrkA-d5:NGF Complexes Cα traces in the TrkB-d5:NT-4/5 complex are shown in cyan (TrkB-d5) and red (NT-4/5), and in the TrkA-d5:NGF complex as biege (TrkA-d5) and blue (NGF). Individual residues shown are Glu-4, Thr-5, Ala-6, Arg-10, and Arg-11 of NT-4/5 (light gray); Ser-2, Ser-3, Pro-5, Ile6, His-8, and Arg-9 of NGF (dark gray); Asp-298, Cys-302, and Cys-345 of TrkB (khaki); and Met-296, Cys-300, and Cys-345 of TrkA (violet). Structure 2001 9, 1191-1199DOI: (10.1016/S0969-2126(01)00681-5)