Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.

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Volume 8, Issue 6, Pages (December 2005)

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Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. A. Western blot analysis shows decreased HMGA2 in H1299 lung cancer cells transfected with the HMGA2 antisense constructs (HMGA2-AS-1 and HMGA2-AS-2) compared with control cells transfected with vector alone. The blot was probed with the HMGA2 antibody as well as an antibody to β-actin to control for protein loading. B. Anchorage-independent growth on soft agar is inhibited in H1299 lung cancer cells transfected with the HMGA2 antisense construct compared with control cells transfected with vector alone. The number of foci formed by H1299 cells transfected with the antisense construct is decreased compared with the control cell transfected with vector control. The experiment was done in duplicate and repeated; results are expressed as an average ± SD from two independent experiments. Photograph of representative foci; bars, 100 μm. C. The growth rates of the control and antisense cells were similar, indicating that the difference in foci formation was not due to inhibition of growth in general. Points, averages from duplicate plates; bars, SD. - - -○- - -, control; —•—, HMGA2 antisense. D. Western blot analysis shows decreased HMGA2 in SK-MES-1 lung cancer cells transfected with the HMGA2 siRNA compared with cells transfected with the control siRNA (siCONTROL nontargeting siRNA pool; Dharmacon). The blot was probed with the HMGA2 antibody as well as an antibody to β-actin to control for protein loading. E. Anchorage-independent growth on soft agar is inhibited in SK-MES-1 lung cancer cells transfected with the HMGA2 siRNA compared with cells transfected with the control siRNA. The number of foci formed by SK-MES-1 cells transfected with the HMGA2 siRNA was decreased compared with cells transfected with the control siRNA. The experiment was done in duplicate and repeated; results are expressed as an average ± SD from two independent experiments. Photograph of representative foci; bars, 100 μm. F. The growth rates of SK-MES-1 cells transfected with either HMGA2 siRNA or control siRNA were similar, indicating that the difference in foci formation was not due to a generalized inhibition of growth or a toxic effect. Points, averages from duplicate plates; bars, SD. - - -○- - -, control siRNA; —•—, HMGA2 siRNA. Francescopaolo Di Cello et al. Mol Cancer Res 2008;6:743-750 ©2008 by American Association for Cancer Research