Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates. Immuno-MS results from antibodies toward 20 different target.

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Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates. Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates.A, immuno-SILAC quantification of 20 target proteins as copy number per cell. The analysis was performed in triplicate, and median values were plotted with error bars showing the standard deviation. B, comparison of copy numbers per cell obtained using immuno-SILAC (y-axis) and PrEST-SILAC (x-axis). C, comparison of protein copy number (y-axis) and transcript abundance from RNA sequencing (x-axis). Fredrik Edfors et al. Mol Cell Proteomics 2014;13:1611-1624 © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.