The Movement of tRNA Through the Ribosome

Slides:

Advertisements

Similar presentations

Figure 1. Mapping entropy distribution through the translation elongation cycle: the LSU is activated first, followed by the SSU. Eight different ribosome.

Advertisements

Relationship between Genotype and Phenotype

Volume 18, Issue 6, Pages (June 2010)

Simulations of HIV Capsid Protein Dimerization Reveal the Effect of Chemistry and Topography on the Mechanism of Hydrophobic Protein Association Naiyin.

Induced Fit and the Entropy of Structural Adaptation in the Complexation of CAP and λ- Repressor with Cognate DNA Sequences Surjit B. Dixit, David Q.

Interaction of Era with the 30S Ribosomal Subunit

Volume 25, Issue 5, Pages (March 2007)

An Approach for De Novo Structure Determination of Dynamic Molecular Assemblies by Electron Cryomicroscopy Bjoern Sander, Monika M. Golas, Reinhard Lührmann,

Volume 16, Issue 5, Pages (May 2008)

Volume 118, Issue 4, Pages (August 2004)

Volume 15, Issue 11, Pages (November 2007)

Volume 88, Issue 1, Pages (January 1997)

Volume 20, Issue 5, Pages (December 2005)

Molecular Model of the Human 26S Proteasome

Volume 6, Issue 5, Pages (November 2000)

Volume 8, Issue 1, Pages (July 2001)

Volume 28, Issue 6, Pages (December 2007)

Volume 93, Issue 7, Pages (June 1998)

Comparison of H+-ATPase and Ca2+-ATPase suggests that a large conformational change initiates P-type ion pump reaction cycles David L. Stokes, Manfred.

Volume 24, Issue 12, Pages (December 2016)

Pawel A. Penczek, Marek Kimmel, Christian M.T. Spahn Structure

Volume 28, Issue 3, Pages (November 2007)

Volume 18, Issue 6, Pages (June 2005)

Locking and Unlocking of Ribosomal Motions

Volume 107, Issue 5, Pages (November 2001)

Electrons and X-rays gang up on the ribosome

Volume 157, Issue 4, Pages (May 2014)

Jingyi Fei, Pallav Kosuri, Daniel D. MacDougall, Ruben L. Gonzalez

Volume 7, Issue 5, Pages (May 2001)

The Path of Messenger RNA through the Ribosome

Volume 27, Issue 2, Pages (July 2007)

Mechanistically Consistent Reduced Models of Synthetic Gene Networks

Volume 57, Issue 3, Pages (February 2015)

Volume 107, Issue 3, Pages (November 2001)

Volume 115, Issue 1, Pages (October 2003)

Volume 22, Issue 8, Pages (August 2014)

Regulation of the Protein-Conducting Channel by a Bound Ribosome

Ribosome Structure and the Mechanism of Translation

Nucleotide Effects on the Structure and Dynamics of Actin

Volume 102, Issue 5, Pages (September 2000)

Volume 129, Issue 5, Pages (June 2007)

Destabilization of the P Site Codon-Anticodon Helix Results from Movement of tRNA into the P/E Hybrid State within the Ribosome Kevin G. McGarry, Sarah.

Jinzhong Lin, Matthieu G. Gagnon, David Bulkley, Thomas A. Steitz Cell

Caught on Camera: Intermediates of Ribosome Recycling

Kenton Abel, Frances Jurnak Structure

Fidelity in protein synthesis

Aurélie Gouron, Anne Milet, Helene Jamet Biophysical Journal

Wei Zhang, Marek Kimmel, Christian M.T. Spahn, Pawel A. Penczek

Molding Atomic Structures into Intermediate- Resolution Cryo-EM Density Maps of Ribosomal Complexes Using Real-Space Refinement Haixiao Gao, Joachim.

Hisashi Ishida, Steven Hayward Biophysical Journal

Mariana Levi, Kien Nguyen, Liah Dukaye, Paul Charles Whitford

Molecular Movement inside the Translational Engine

Mapping the Position of Translational Elongation Factor EF-G in the Ribosome by Directed Hydroxyl Radical Probing Kevin S Wilson, Harry F Noller Cell

Dynamics of tRNA at Different Levels of Hydration

Penton Release from P22 Heat-Expanded Capsids Suggests Importance of Stabilizing Penton-Hexon Interactions during Capsid Maturation Carolyn M. Teschke,

Volume 8, Issue 1, Pages (July 2014)

Volume 121, Issue 5, Pages (June 2005)

Volume 26, Issue 1, Pages (April 2007)

Volume 26, Issue 3, Pages e3 (March 2018)

Volume 44, Issue 2, Pages (October 2011)

Mediha Esra Altuntop, Cindy Tu Ly, Yuhong Wang Biophysical Journal

Molecular Structures of Transcribing RNA Polymerase I

Solution Structure of the E. coli 70S Ribosome at 11.5 Å Resolution

OmpT: Molecular Dynamics Simulations of an Outer Membrane Enzyme

The ribosome – a macromolecular machine par excellence

Volume 32, Issue 2, Pages (October 2008)

Volume 100, Issue 3, Pages (February 2000)

A method for differentiating proteins from nucleic acids in intermediate-resolution density maps: cryo-electron microscopy defines the quaternary structure.

Volume 113, Issue 6, Pages (June 2003)

Ribosomal translocation: EF-G turns the crank

Presentation transcript:

The Movement of tRNA Through the Ribosome Joachim Frank, Rajendra K. Agrawal Biophysical Journal Volume 74, Issue 1, Pages 589-594 (January 1998) DOI: 10.1016/S0006-3495(98)77817-8 Copyright © 1998 The Biophysical Society Terms and Conditions

Figure 1 Evidence of a close fit between the shapes of the intersubunit space and the tRNA molecule. (a) Reconstruction of the ribosome, using a contour that encloses a molecular volume of 4×106Å3. (b) Surface representation of tRNA in an orientation assumed to be likely within the ribosome. AC, anticodon loop; CCA, acceptor end. (c) Cross section through the ribosome reconstruction in the same orientation as depicted in the top panel. The outline of tRNA (b) has been superimposed in green. Landmarks: Small subunit: h, head; sp, spur; cl, cleft. Large subunit: CP, central protuberance; St, L7/L12 stalk; IC, interface canyon. Biophysical Journal 1998 74, 589-594DOI: (10.1016/S0006-3495(98)77817-8) Copyright © 1998 The Biophysical Society Terms and Conditions

Figure 2 Topography of the intersubunit space. The ribosome was separated into its two subunits by a cutting plane that roughly divides the intersubunit space in half. Landmarks: small subunit: h, head; sp, spur; cl, cleft. Large subunit: L1, L1 protein; St, L7/L12 stalk; IC, interface canyon; CP, central protuberance. Joint features: CR, central ridge; FB, factor binding region. Biophysical Journal 1998 74, 589-594DOI: (10.1016/S0006-3495(98)77817-8) Copyright © 1998 The Biophysical Society Terms and Conditions

Figure 3 Three main stages of the elongation cycle following the hybrid-site model, shown by using a transparent model of the 70S ribosome (seen from top). (Left) Pretranslocational state, with tRNAs in the A and P sites. (Middle) Hybrid or transitional state, with one tRNA in the P/E state, the other in the A/P state. (Right) Posttranslocational state in which the ribosome is ready for a new ternary complex, aa-tRNA · EF-Tu · GTP, depicting one tRNA in the P site, the other in the E site. (Positions of A and E sites are adopted from Agrawal et al. (1996).) Landmarks in the left-hand panel are defined as in Fig. 2. Biophysical Journal 1998 74, 589-594DOI: (10.1016/S0006-3495(98)77817-8) Copyright © 1998 The Biophysical Society Terms and Conditions