Epidermal Langerhans Cell Depletion After Artificial Ultraviolet B Irradiation of Human Skin In Vivo: Apoptosis Versus Migration  Wendy Kölgen, Hilde Both, Huib van Weelden, Kees L.H. Guikers, Carla A.F.M. Bruijnzeel-Koomen, Edward F. Knol, Willem A. van Vloten  Journal of Investigative Dermatology  Volume 118, Issue 5, Pages 812-817 (May 2002) DOI: 10.1046/j.1523-1747.2002.01742.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Double staining of CD1a (blue) and active-caspase 3 (red) of buttock skin, unexposed (– UV), 24 h (+ UV + 24 h) and 48 h after UVB exposure (+ UV + 48 h). Scale bar: 50 µm. Journal of Investigative Dermatology 2002 118, 812-817DOI: (10.1046/j.1523-1747.2002.01742.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Number of CD1a+ Langerhans cells (mean ± SEM) in blister roofs (bullet) and blister fluid (CD1a+ cells, ▪ and CD1a+/H3+ cells, ▴, of arm skin, unexposed (0 h) and UVB-exposed (18 h, 24 h, 30 h, 42 h, 48 h). Average n per time-point = 5. Journal of Investigative Dermatology 2002 118, 812-817DOI: (10.1046/j.1523-1747.2002.01742.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Double staining of CD1a (blue) and H3 (red) of buttock skin, unexposed (– UV) and 24 h after UVB exposure (+ UV) with an example of a doubled stained cell, migrating out of the epidermis (black arrow). Scale bar: 50 µm. Journal of Investigative Dermatology 2002 118, 812-817DOI: (10.1046/j.1523-1747.2002.01742.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Double staining of CD1a (blue) and H3 (red) in blister fluid of the UVB-exposed skin. Journal of Investigative Dermatology 2002 118, 812-817DOI: (10.1046/j.1523-1747.2002.01742.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions