What is the major issue of this paper?

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Presentation transcript:

What is the major issue of this paper? How C. elegans embryos divide? Whether gut differentiation needs inductive signals? How to separate C. elegans blastomeres? How to observe gut differentiation Whether gut differentiation nees local determinants?

What is the major approach that the author used to address his major question? Antibody staining Separation and reconstitution of blastomeres C. elegans lineage analysis Multiple approaches are used and needed. Genetic analysis

Which of the following is important for inductive gut differentiation in C. elegans? ABa ABa + ABp MS P2 ABp + P2

Which of the following is a correct statement? Cytoplasmic determinant is not important for gut differentiation The inductive interaction between EMS and P2 occurs shortly after cell division Recombining isolated EMS and P2 always results in gut differentiation Recombining isolated EMS and ABx can not yield correct gut differentiation Isolated EMS could not produce gut

What is the most important lesson that you learn from reading this paper? It is a very cool paper Inductive signal is important for C. elegans gut differentiation One can always question existing or established dogma C. elegans is a good animal model for studying embryo development You need to have very good hands to be a good scientist