Todd K. Rosengart, M. D. , John P. Kupferschmid, M. D. , Victor J

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Presentation transcript:

Heparin-binding growth factor-I (endothelial cell growth factor) binds to endothelium in vivo Todd K. Rosengart, M.D., John P. Kupferschmid, M.D., Victor J. Ferrans, M.D., Ward Casscells, M.D., Thomas Maciag, Ph.D., Richard E. Clark, M.D. Journal of Vascular Surgery Volume 7, Issue 2, Pages 311-317 (February 1988) DOI: 10.1016/0741-5214(88)90150-4 Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Binding of 125I-labeled HBGF-I to aortic endothelium as a function of HBGF-I concentration. Points represent mean ± standard error, insert represents Scatchard plot analysis of data as determined by computer-assisted best fit. Histogram on graph labeled “cold” represents 125I-labeled HBGF-I binding after injection of twentyfold excess unlabeled HBGF-I (200 ng 125I-labeled HBGF-I injected). Journal of Vascular Surgery 1988 7, 311-317DOI: (10.1016/0741-5214(88)90150-4) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Plot of 125I-labeled HBGF-I binding to uninjured (right) rat carotid artery after injection of 125I-labeled HBGF-I with and without heparin (2.5 U/ng protein). Also shown are averages with standard error. Journal of Vascular Surgery 1988 7, 311-317DOI: (10.1016/0741-5214(88)90150-4) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Plot of 125I-labeled HBGF-I binding to injured vs. uninjured carotid arteries (left vs. right) in the same rat (indicated by connecting line). Also shown are averages with standard error. Journal of Vascular Surgery 1988 7, 311-317DOI: (10.1016/0741-5214(88)90150-4) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Plot of 125I-labeled HBGF-I binding to injured (left) carotid artery after injection of 125I-labeled HBGF-I with and without heparin (2.5 U/ng protein). Also shown are averages with standard error. Journal of Vascular Surgery 1988 7, 311-317DOI: (10.1016/0741-5214(88)90150-4) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions