Volume 114, Issue 6, Pages (June 1998)

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Volume 114, Issue 6, Pages 1211-1220 (June 1998) Expression of transforming growth factor β receptors in normal human colon and sporadic adenocarcinomas Rally Eskinazi, Anne Resibois, Michal Svoboda, Marie-Odile Peny, Michael Adler, Patrick Robberecht, Jean-Luc van Laethem Gastroenterology Volume 114, Issue 6, Pages 1211-1220 (June 1998) DOI: 10.1016/S0016-5085(98)70427-5 Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 (A) Immunoblotting for RI on cell fractions of normal colon (NORM.) and colon tumor (TUMOR). The specific band corresponds to 55 kilodaltons. M, membrane fraction; N, nuclear fraction; C, cytoplasmic fraction. (B) Immunoblotting for RII on cell fractions with (2 μg/mL) or without (−) preincubation with the antigenic peptide. The specific band corresponds to 60 kilodaltons. Mb, membranous fraction. Lane 1, normal colon membrane fraction (N); lane 2, tumoral colon membrane fraction (T); lane 3, normal colon membrane fraction (N); lane 4, tumoral colon membrane fraction (T); lane 5, normal colon cytosolic fraction (N); lane 6, tumoral colon cytosolic fraction (T); lane 7, normal colon cytosolic fraction (N); lane 8, tumoral colon cytosolic fraction (T); and lane 9, tumoral colon membrane fraction (T). Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 1 (A) Immunoblotting for RI on cell fractions of normal colon (NORM.) and colon tumor (TUMOR). The specific band corresponds to 55 kilodaltons. M, membrane fraction; N, nuclear fraction; C, cytoplasmic fraction. (B) Immunoblotting for RII on cell fractions with (2 μg/mL) or without (−) preincubation with the antigenic peptide. The specific band corresponds to 60 kilodaltons. Mb, membranous fraction. Lane 1, normal colon membrane fraction (N); lane 2, tumoral colon membrane fraction (T); lane 3, normal colon membrane fraction (N); lane 4, tumoral colon membrane fraction (T); lane 5, normal colon cytosolic fraction (N); lane 6, tumoral colon cytosolic fraction (T); lane 7, normal colon cytosolic fraction (N); lane 8, tumoral colon cytosolic fraction (T); and lane 9, tumoral colon membrane fraction (T). Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 2 Thermocycling sequencing analysis of (A) the polyA tract and (B) the polyGT tract. No mutations were observed in either microsatellite-like region in any of the tumoral or normal samples. Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 2 Thermocycling sequencing analysis of (A) the polyA tract and (B) the polyGT tract. No mutations were observed in either microsatellite-like region in any of the tumoral or normal samples. Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 3 Immunostaining for the TGF-β RII in normal mucosa. The staining is located at the luminal surface and is absent in the deeper layers. The staining for TGF-β RI was similar (original magnification 260×). L, lumen. Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 4 High-power magnification of a triangular neuroendocrine cell (arrowhead) in the deeper layers of the mucosa stained with the RII antibody. The same cell was stained with the RI antibody and the antichromogranin A antibody (original magnification 340×). Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 5 Staining with RI antibody that was preincubated overnight with the control peptide. The field shown corresponds to that of Figure 7 and is free of immunoreactivity (original magnification 125×). Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 6 RII immunoreactivity of a neoplastic cluster (T). The adjacent normal-looking mucosa is much less reactive, if at all (N) (original magnification 260×). Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 7 RI immunoreactivity localized throughout the neoplastic glands (original magnification 125×). Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions

Fig. 8 RII immunoreactivity showing a patchy distribution of the staining between the groups of cells and within the same epithelial cluster (original magnification 240×). Gastroenterology 1998 114, 1211-1220DOI: (10.1016/S0016-5085(98)70427-5) Copyright © 1998 American Gastroenterological Association Terms and Conditions