Diagnostic value of an ELISA using a recombinant 54-kDa species-specific protein from Chlamydia pneumoniae J-M. Sueur, K. Beaumont, T. Cabioch, J. Orfila,

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Diagnostic value of an ELISA using a recombinant 54-kDa species-specific protein from Chlamydia pneumoniae J-M. Sueur, K. Beaumont, T. Cabioch, J. Orfila, F. Betsou Clinical Microbiology and Infection Volume 12, Issue 5, Pages 470-477 (May 2006) DOI: 10.1111/j.1469-0691.2006.01390.x Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Immunoblots of (1) Chlamydia pneumoniae elementary bodies, (2) C. pneumoniae outer-membrane complex, (3) P54 protein, (4) P54 aa 184–344, and (5) P54 aa 1–183 with (A) monoclonal anti-V5 antibody or (B) polyclonal anti-P54 serum. Molecular size markers correspond to 14 800, 28 201, 41 603, 55 004 and 68 406 Da, respectively. Clinical Microbiology and Infection 2006 12, 470-477DOI: (10.1111/j.1469-0691.2006.01390.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Correlation between outer-membrane complex (OMC)-specific antibodies and P54-specific IgG antibodies. Clinical Microbiology and Infection 2006 12, 470-477DOI: (10.1111/j.1469-0691.2006.01390.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Comparison of the active site region of Morganella morganii histidine decarboxylase (HDC), Klebsiella pneumoniae HDC, Vibrio anguillarum HDC, Escherichia coli X-his dipeptidase and Chlamydia pneumoniae P54. Residues conserved in the five sequences are marked by a double asterisk. Residues conserved in at least two sequences are marked by a single asterisk. Similar residues are marked by double dots. The pyridoxal-P-binding lysine is marked by an exclamation point. Clinical Microbiology and Infection 2006 12, 470-477DOI: (10.1111/j.1469-0691.2006.01390.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 4 (A) ELISA reactivities of polyclonal antisera from mice immunised with P54, P54 aa 1–183 and P54 aa 184–344 against homologous antigens. (B) ELISA of polyclonal antisera from mice immunised with P54 against P54 protein, P54 aa 1–183 and P54 aa 184–344. Clinical Microbiology and Infection 2006 12, 470-477DOI: (10.1111/j.1469-0691.2006.01390.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 5 Dot-blots with (1) Chlamydia pneumoniae elementary bodies (EBs), (2) C. pneumoniae outer-membrane complex (OMC), (3) P54, (4) P54 N-terminal fragment, and (5) P54 central fragment. Dot-blots were incubated with sera from mice infected with C. pneumoniae or immunised with C. pneumoniae antigens. (A) sera from mice infected with C. pneumoniae, 22 days after infection; (B) sera from mice infected with C. pneumoniae, 50 days after infection; (C) sera from mice immunised with C. pneumoniae EBs; (D) sera from mice immunised with C. pneumoniae OMC; (E) sera from mice immunised with P54; (F) sera from control mice. Clinical Microbiology and Infection 2006 12, 470-477DOI: (10.1111/j.1469-0691.2006.01390.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions