Assembly of the novel bat gammaherpesvirus genome highlights ORFs related to EHV-2 and accessory ORFs related to other gammaherpesviruses. Assembly of.

Slides:

Advertisements

Similar presentations

Identification of obesity-associated intergenic long noncoding RNAs

Advertisements

What is comparative genomics? Analyzing & comparing genetic material from different species to study evolution, gene function, and inherited disease Understand.

SRB Genome Assembly and Analysis From 454 Sequences HC70AL S Brandon Le & Min Chen.

454 Genome Sequence Assembly and Analysis HC70AL S Brandon Le & Min Chen.

Engineering magnetosomes to express novel proteins Which ones? Tweaking p18 Linker Deleting or replacing GFP Something else? TRZN Oxalate decarboxylases.

Down Syndrome: Genes, Model Systems, and Progress towards Pharmacotherapies and Clinical Trials for Cognitive Deficits Cytogenet Genome Res 2013;141:

Figure 2 Genetic organization and translation of hepatitis E virus

PCR genotype analysis to determine RNP-mediated knockout efficiency in C. lusitaniae. PCR genotype analysis to determine RNP-mediated knockout efficiency.

Today… Review a few items from last class

Schematic view of SGI1-L and its specific features encountered in Morganella morganii strain LIM90. Schematic view of SGI1-L and its specific features.

A User’s Guide to GO: Structural and Functional Annotation

Schematic representation of the MAT loci of S. pombe, P. jirovecii, P

Homology network of 4,131 putative oomycete RxLR effectors.

The ability of the SOP to sequence and identify unknown samples.

Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae phages. Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae.

Genus-level genomic OTU (gOTU) richness.

Genomic rearrangements of E

Schematic representation of the lbk gene, positions of gRNAs, and knock-in cassette. Schematic representation of the lbk gene, positions of gRNAs, and.

The Release 5.1 Annotation of Drosophila melanogaster Heterochromatin

Genomic context, sequence identity, and structural homology modeling of the aadA31 gene detected in P. multocida PM13 and H. somni HS31. Genomic context,

Venn diagram of blaKPC-containing plasmids detected in the environment and patients. Venn diagram of blaKPC-containing plasmids detected in the environment.

Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1,

Genomes with Fe-S cluster assembly-related genes.

Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. (A) progressiveMauve.

Isolation and phylogenetic characterization of ISS strains.

Comparison of the variable regions of (A) pHNZY32, pHNZY118, and pHNAH24; (B) pHNMCC14; (C) pHNFKU92; (D) pE80; (E) pECB11; (F) p42-2; and (G) pSLK172-2.

Expression response of AT-rich genes to lanthanides.

Flavivirus particle assembly

Polymorphism discovery in 09-CB1 × IPO323 versus 09-ASA-3apz × IPO94269 bulks. Polymorphism discovery in 09-CB1 × IPO323 versus 09-ASA-3apz × IPO94269.

The human norovirus genome.

Pearson correlation of gene expression identifies distinct groups of male- and female-enriched genes. Pearson correlation of gene expression identifies.

Phylogenetic tree of 170 Lactobacillus species.

Comparison of gene expression maps from available S

(A, left) Radial cladogram based on RAxML-based maximum-likelihood phylogeny (500 bootstraps, gamma distribution model, and LG+F substitution model) constructed.

(A) Scale map of connections between contigs in Ver_v2 suggested by the alignment of paired-end Illumina reads (insert size, ~300 bp). (A) Scale map of.

Histidine kinases are expanded in the fusaria.

Transcriptomic analysis of Jamaican fruit bats infected with Tacaribe virus (TCRV). Transcriptomic analysis of Jamaican fruit bats infected with Tacaribe.

.1Sources of DNA and Sequencing Methods 2 Genome Assembly Strategy and Characterization 3 Gene Prediction and Annotation 4 Genome Structure 5 Genome.

Transcript length distribution resulting from different assemblies of the embryo samples across the three technologies (HiSeq, MiSeq, and PacBio). Transcript.

Bioinformatics analysis of the small TbTim amino acid sequences.

Comparison of N. gonorrhoeae gene expression in infected men in vivo and isolates grown in vitro. Comparison of N. gonorrhoeae gene expression in infected.

Volume 10, Issue 2, Pages (August 2011)

Maximum likelihood phylogeny of USA500 and other CC8 strains.

S. pneumoniae GapA is sensitive to oxygen and H2O2 exposure.

Schematic illustration of polysaccharide capsule biosynthesis (A) and nucleotide/cofactor biosynthesis (B) in S. pneumoniae D39 and its relationship to.

RNA-Seq analysis of CYR1 cells in the adaptation to acid pH.

Deletion of the multicopy A2 family genes and increased efficiency through coselection for parasites with CRISPR-Cas9 activity. Deletion of the multicopy.

C. difficile colonization alters gene transcription of taxonomic groups differentially between antibiotic pretreatments. C. difficile colonization alters.

Nonmetric multidimensional scaling (NMDS) (A) and dendrogram and heat map (B) of the relative abundances of the data in the custom database across the.

General structure of RIFINs and STEVORs

The Amazon viral scaffolds and viral genomes most important for river and plume segregation. The Amazon viral scaffolds and viral genomes most important.

Phenotypic and genotypic features of bacteriophage Andhra.

Editing and reconstitution of CPAR2_

Schematic diagram of the JCV genome.

Phylogenetic analysis of K. quasipneumoniae subsp

Uptake of amino acids by C. albicans.

ITS rRNA gene locus. ITS rRNA gene locus. Schematic of the eukaryotic ribosomal gene cluster. The SILVA database contains sequences of the 18S gene, while.

Break-induced marker excision concept.

Comparison of community fungal and bacterial/archaeal diversity levels

Distribution of the diversity numbers of antibiotic resistance and virulence factor protein families by environmental metagenomes’ protein family richness.

Adam T. McGeoch, Stephen D. Bell Cell

RNA-seq analysis from the MVI-it culture indicates that a majority of the BGHV8 coding sequences are transcribed. RNA-seq analysis from the MVI-it culture.

Formation of complex antibiotic resistance regions.

Transient CRISPR-Cas9 system.

Maps of Structures of the Ruby Locus in the Different Citrus Species, Orange Accessions, and Hybrids.Thick green arrows, retrotransposon LTRs; thick gray.

Phylogenetic analysis of complete Fusobacterium genomes.

Analysis of Fusobacterium nucleatum ATCC genomes compared to previous builds. Analysis of Fusobacterium nucleatum ATCC genomes compared to.

Characterization of SFV-specific small RNAs bound by Piwi4 and Ago2 in Aag2 cells. Characterization of SFV-specific small RNAs bound by Piwi4 and Ago2.

Potent and sequence-specific removal through genome targeting with CRISPR-Cas systems. Potent and sequence-specific removal through genome targeting with.

Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a.

Presentation transcript:

Assembly of the novel bat gammaherpesvirus genome highlights ORFs related to EHV-2 and accessory ORFs related to other gammaherpesviruses. Assembly of the novel bat gammaherpesvirus genome highlights ORFs related to EHV-2 and accessory ORFs related to other gammaherpesviruses. To assemble a single contig of 129,563 bp, contigs assembled with 100-bp reads from Illumina HiSeq were joined with 270-bp reads from Illumina MiSeq data. The genome was annotated using the Viral Genome ORF Reader (VIGOR). Open reading frames are highlighted as follows: yellow, ORFs with homology to EHV-2; blue, ORFs with homology to other herpesvirus proteins; black, repeat regions; gray, putative LANA gene with an internal unresolved repeat region. ncRNA, noncoding RNA. Reed S. Shabman et al. mSphere 2016; doi:10.1128/mSphere.00070-15