The importance of strain variation in virulence of Candida dubliniensis and Candida albicans: results of a blinded histopathological study of invasive.

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Presentation transcript:

The importance of strain variation in virulence of Candida dubliniensis and Candida albicans: results of a blinded histopathological study of invasive candidiasis L.R. Ásmundsdóttir, H. Erlendsdóttir, B.A. Agnarsson, M. Gottfredsson Clinical Microbiology and Infection Volume 15, Issue 6, Pages 576-585 (June 2009) DOI: 10.1111/j.1469-0691.2009.02840.x Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1. Quantification of tissue burden of organisms and inflammatory responses in kidneys of animals infected with Candida albicans and C. dubliniensis. A scoring system of 0–4 for each parameter was developed. For quantification of fungal load, kidney slices were stained by methenamine-silver stain and scored as 0 (if no yeasts were visible) or as 1, 2, 3 or 4 (figures a, b, c and d, respectively). For estimation of inflammatory responses, slices were stained with haematoxylin-eosin stain (H&E) and the amount of inflammatory cells was scored in the same way as fungal load (figures e, f, g and h, respectively). Magnification 310×. Clinical Microbiology and Infection 2009 15, 576-585DOI: (10.1111/j.1469-0691.2009.02840.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2. Phylogenetic relationships of the Candida isolates used in this study as determined by PCR fingerprinting. Combined results from PCR with four single primers [M13; (GACA)4; PA03; T3B] are shown. Unweighted pair-group method using average linkages and the Dice coefficient were used to construct the dendrogram. The genotypes of the C. dubliniensis isolates are given on the right. *Isolate IS-124 yielded an amplimer with both genotype 1- and genotype 2-specific primer pairs. Clinical Microbiology and Infection 2009 15, 576-585DOI: (10.1111/j.1469-0691.2009.02840.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3. Survival of mice infected by Candida albicans (three isolates, n = 99) compared with C. dubliniensis (nine isolates, n = 157). Survival was monitored three times daily for 1 week. Other C. dubliniensis strains: IS-124; IS-176; CD-541; 1329; 1862; and CBS 8500. Clinical Microbiology and Infection 2009 15, 576-585DOI: (10.1111/j.1469-0691.2009.02840.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions