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(apoptotic + necrotic) Untreated hIgG (crosslinked) Dacetuzumab (soluble) (crosslinked) Cell Death (Ramos) Percent Cell Death (apoptotic + necrotic) Dacetuzumab (crosslinked) Proliferation (Ramos) ng/ml b Dacetuzumab Signaling (RL) c phospho-ERK1/2 MAPK b-Actin 111 ng/ml Dacetuzumab Untreated 333 1.00x103 37.0 12.3 4.12 Ramos Dacetuzumab (soluble) (crosslinked) 1 3 10 20 Untreated hIgG (soluble) hIgG (crosslinked) Crosslinking Ab CD40 Ligand mg/ml phospho-AKT phospho-ERK1/2 MAPK IkB-a phospho-p38 MAPK Lane: 2 4 5 6 7 8 9 11 Supplementary Figure 1 Lewis, et al.