Obesity-Blocking Neurons in Drosophila

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Obesity-Blocking Neurons in Drosophila Bader Al-Anzi, Viveca Sapin, Christopher Waters, Kai Zinn, Robert J. Wyman, Seymour Benzer Neuron Volume 64, Issue 2, Pages 290-291 (October 2009) DOI: 10.1016/j.neuron.2009.10.002 Copyright © 2009 Elsevier Inc. Terms and Conditions

Figure 2 The c673a-Gal4 Neurons Overlap Only Minimally with the Fru-Gal4 Neurons c673a-Gal4/2xUAS-GFP brain (A) showing the neurosecretory cluster (arrow), mushroom body (asterisks), and the ventral ganglion (B). Fru-Gal4/2xUAS-GFP brain (C) showing the neurosecretory cluster (arrow), mushroom body (asterisks), and the ventral ganglion (D). Anti-FruM (red in E and F) rarely costained the green c673a-Gal4/UAS-nuc-GFP neurons (green in E and F), with overlap occurring in only few yellow neurons in the neurosecretory cluster and subesophageal ganglion (E and F, arrows). Anti-DILP immunostaining (red in G and H) of c673a-Gal4/2xUAS-GFP neurons (green in G) and of Fru-Gal4/2xUAS-GFP neurons (green in H) indicates that most of the insulin expressing (DILP) neurons are both c673a-Gal4 and Fru-Gal4 positive (yellow in G and H, arrows). Immunostaining with the dopaminergic marker anti-tyrosine hydroxylase (red in I–L) show that most are also c673a-Gal4/2xUAS-GFP neurons (yellow in I and J, arrows), however, only few are Fru-Gal4/2xUAS-GFP positive neurons (yellow in K and L, arrows). Anti-serotonin immunostaining (red in M–P) shows little overlap between serotonergic neurons and c673a-Gal4/2xUAS-GFP (green in M and N) and Fru-Gal4/2xUAS-GFP neurons (green in O and P). However, few c673a-Gal4 and Fru-Gal4 neurons in the subesophageal and ventral ganglion are serotonergic (yellow in M–P, arrows). Silencing of the few neurons shared between c673a-Gal4 and Fru-Gal4 does not affect fat store level. UAS-Shits silencing of dopaminergic neurons using TH-Gal4 (Q), serotonergic neurons using Eg-Gal4 (R), insulin expressing neurons by using Dilp2-Gal4 (S), or both dopaminergic and serotonergic neurons using DDC-Gal4 (T) does not produce changes in fly fat content compared to control flies with single transgenes as examined by the TLC assay. Arrows in TLC panels point to butter standard lanes. Neuron 2009 64, 290-291DOI: (10.1016/j.neuron.2009.10.002) Copyright © 2009 Elsevier Inc. Terms and Conditions