Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6)‏ Computational wound healing gradients and in vivo.

Slides:



Advertisements
Similar presentations
Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.
Advertisements

Microtubule perturbations cause Ste5 patches to form less reliably, delay patch formation, and cause patches to persist for less time Microtubule perturbations.
DNase‐HS sites are main independent determinants of DNA replication timing Simulations based on genome sequence features (GC content, CpG islands), or.
Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.
Analysis of mutational effects of parameters on the phenotype (i. e
Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.
Predicted and measured double‐ring formation.
Functional analysis of duplicate pair CIK1–VIK1 (A) Genetic interaction profile similarity. Functional analysis of duplicate pair CIK1–VIK1 (A) Genetic.
Clonal expansion of sporadically induced K‐rasG12D in Lgr5hi cells Confocal scanning of the bottom of small intestinal crypts at indicated time points.
Dual allele labeling reveals a trans‐acting source for extrinsic noise
Notch levels in niche cells
The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.
Skin proliferation kinetics and in vivo fibroblast lineage tracing during dermal maturation (related to Fig 4)‏ Skin proliferation kinetics and in vivo.
Dose response of pAGA1 and pFIG1 induction
Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.
cuf2Δ and fzr1Δ cells show a defect in meiotic termination.
Characterization of promoters relative to pAGA1
Dynamics and variability of SMAD2 signaling in single cells
Hierarchical structure in the yeast transcription regulatory network.
Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),
TFEB stimulates lysosomal–autophagosomal fusion and clearance
Heat maps showing global relative growth phenotype and comparison between measured and predicted values. Heat maps showing global relative growth phenotype.
Dermal architecture is defined by an inverse correlation between fibroblast proliferation and ECM deposition Dermal architecture is defined by an inverse.
Alignment time for Clustal Omega (red), MAFFT (blue), MUSCLE (green) and Kalign (purple) against the number of sequences of HomFam test sets. Alignment.
Mutants of the Group III have differentially impaired induction of pAGA1 and pFIG1 Mutants of the Group III have differentially impaired induction of pAGA1.
Dynamic map of the Nap1p/Kcc4p interaction.
The limiting, degrading entity hypothesis
Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+
Fibroblast proliferation and ECM changes during wound healing
ClpX facilitates FtsZ depletion during carbon starvation
Transcriptomic and epigenetic changes associated with Factor 1 in the scMT data Transcriptomic and epigenetic changes associated with Factor 1 in the scMT.
Comparison of nuclear surface area of HeLa and RKO cells
Live‐cell imaging uncovers a slowly cycling drug‐resistant state involved in adaptation to RAF inhibition Live‐cell imaging uncovers a slowly cycling drug‐resistant.
Development of a 3D tissue model and in vivo live imaging during dermal maturation Development of a 3D tissue model and in vivo live imaging during dermal.
Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.
Design and optimization of the computational model.
Large‐scale image‐based CRISPR‐Cas9 gene perturbation profiling
Proliferative advantage depends on environmental dynamics
Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.
Effect of HDAC inhibition on the steady‐state and dynamic transcriptional response and associated noise. Related to Fig 7 Effect of HDAC inhibition on.
Loss of tight junctions on Rac1 activation in mature cysts.
Protein interactions at the nuclear pore.
Melting behavior of protein complexes
Characterising dermis expansion and gene expression changes during mouse development (related to Fig 1)‏ Characterising dermis expansion and gene expression.
In vivo imaging of the wound bed at later time points (related to Fig 7)‏ In vivo imaging of the wound bed at later time points (related to Fig 7) A–CRepresentative.
Nedd4 proteins. Nedd4 proteins. (A) Schematic representation of rNedd4‐1, hNedd4‐1 and hNedd4‐2 (not to scale), with % amino‐acid identity between them.
Experimental validation of predicted endocytosis functions in human.
Global and focused views of human interaction map.
PDE1 predominantly regulates the threshold for RP induction.
Remodeling of the mitochondrial and extracellular proteome during Caenorhabditis elegans aging Remodeling of the mitochondrial and extracellular proteome.
Patterns and regulation of age‐related splicing changes.
Time‐course analysis of NICD degradation.
3D model and in vivo live imaging of dermal wound repair
Integration of proteomic data into the model
The transcriptional behavior of GREB1 changes with estrogen dose and exhibits considerable cell‐to‐cell variation (see also Fig EV2 and Movie EV2)‏ The.
DIA1(R1204X) induces elongated and thick microvilli in HeLa cells
Mpdz was localized proximal to the apical surface of CPECs
PhoB also translocates to plant nuclei in leaf and crown cells in response to HK activation with exogenous cytokinin. PhoB also translocates to plant nuclei.
Inhibitory effect of perhexiline on the proliferation of the HepG2 cell line A, BPerhexiline was used to mimic the effect of the l‐carnitine analog on.
Kinetics of GREB1 induction by estrogen in single cells (see also Fig EV4 and Movie EV3)‏ Kinetics of GREB1 induction by estrogen in single cells (see.
Single‐molecule dynamics of the active mutant, hDIA1(R1204X), in XTC cells Single‐molecule dynamics of the active mutant, hDIA1(R1204X), in XTC cells A–DGFP‐tagged.
MiR‐133 enhances generation of functional iCMs ATime course of αMHC‐GFP and cTnT expression by GMT or GMT/miR‐133 transduction in MEFs. See also Supplementary.
Topologies, synthetic implementations and expression profiles of the networks studied Topologies, synthetic implementations and expression profiles of.
Contribution of amino‐acid production to the catabolism of BCAAs and AAAs, and to ATP generation under slow‐growing conditions. Contribution of amino‐acid.
Growth factor‐stimulated ERK kinase activities in the cytosol and nucleus are kinetically ordered and do not show dramatic adaptation Growth factor‐stimulated.
CRISPR‐Cas9 gene perturbation profiling in HeLa cells
Changes in fibroblast activation and proliferation outside the wound and CHP staining in the wound bed (related to Fig 5)‏ Changes in fibroblast activation.
BRI1 signaling at the dividing cells restores overall root growth
Nutrient availability regulates growth of the X. laevis optic tectum.
Inferred promoter–metabolite regulation network (Table EV7)
Presentation transcript:

Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6)‏ Computational wound healing gradients and in vivo imaging of the wound bed at PW2 (related to Fig 6) Gradients used in the computational model. Top panel represents the wound healing gradient during the course of the simulation. Bottom panel represents the epidermal gradient in the vicinity of the wound during the course of the simulation.Representative time‐lapse images of adult PDGFRαH2BEGFP dermal fibroblasts (green) and collagenous extracellular matrix shown with second harmonic generation (SHG) in purple 2 days after wounding. Dotted line indicates wound edge. Note that fibroblasts are not actively migrating at 2 days postwounding. Please refer to associated Movie EV5. Scale bar, 100 μm. Emanuel Rognoni et al. Mol Syst Biol 2018;14:e8174 © as stated in the article, figure or figure legend